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Analysis Output/Output Files/Gene Expression File/Differential Expression Section

Differential Expression Section

The Differential Expression section consists of one table for each pair of samples. The information in this section is based on DESeq2 results and information in the input manifest. Each table has the following fields.

Column Heading

Description

Gene Name

The name of the gene.

Amplicon ID

The amplicon identifier constructed from the gene name, transcript ID, left exon, right exon, and assay ID.

Target ID

The unique identifier for the target.

Control Group

The control sample group.

Comparison Group

The comparison sample group.

Control Raw Mean Count

The mean of counts for control samples.

Comparison Raw Mean Count

The mean of counts for comparison samples.

Control Normalized Mean Count

The control raw mean count with library size normalization.

Comparison Normalized Mean Count

The comparison raw mean count with library size normalization.

Fold Change

The ratio of Comparison Normalized Mean Count divided by Control Normalized Mean Count.

Log2(Fold Change)

The log-scaled fold change.

P-value

The statistical significance of the differential expression.

Q-value

The p-value adjusted for false discovery rate (FDR).

Transcript

Transcript targeted by assay.

Exons

Left and right exons of targeted region (if available).

Target Type

The type of amplicon.