Introduction

The UMI Error Correction BaseSpace^™ Sequence Hub App analyzes sequencing reads from DNA libraries prepared using the TruSight^™ Oncology UMI Reagents. Unique Molecular Identifiers (UMIs) are short sequences of bases added at the beginning of library preparation that can be used to uniquely identify each molecule going into the reaction. The UMI Error Correction BaseSpace Sequence Hub App uses the UMI information contained within the sequencing reads to collapse PCR duplicates originating from the same unique DNA fragment. Collapsing PCR duplicates results in accurate consensus reads without losing the signal of very low frequency sequence variations.

The UMI Error Correction BaseSpace Sequence Hub App produces three BAM files per sample:

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BAM file with aligned and noncollapsed sequencing reads

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BAM file with aligned and collapsed sequencing reads.

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BAM file with aligned, collapsed, and stitched sequencing reads

The UMI Error Correction BaseSpace Sequence Hub App also produces a report containing read collapsing metrics and intermediate analysis files. Analysis of up to 48 samples is supported. For Research Use Only. Not for use in diagnostic procedures.