Create an AmpliSeq for Illumina Hotspot Design/Configure an AmpliSeq for Illumina Hotspot Design

Configure an AmpliSeq for Illumina Hotspot Design

1. Select a Sample Type.


DNA from high-quality FFPE tissue can be used with longer amplicon designs. However, uniformity and representation of longer amplicons can decrease.

2. Select the Max Amplicon Length.

375 bp is recommended for MiSeq only.

3. [Optional] Select Learn more about stringency for information on how different stringency levels impact the final assay design.
4. Select a Stringency from the drop-down list.

Stringency represents the strictness with which probes and targets hybridize. The higher the stringency is set, the more complimentary the probe and target must be to hybridize.

5. Select Next.