Configure an AmpliSeq for Illumina Hotspot Design
NOTE
DNA from high-quality FFPE tissue can be used with longer amplicon designs. However, uniformity and representation of longer amplicons can decrease.
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2.
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Select the Max Amplicon Length. |
375 bp is recommended for MiSeq only.
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3.
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[Optional] Select Learn more about stringency for information on how different stringency levels impact the final assay design. |
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4.
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Select a Stringency from the drop-down list. |
Stringency represents the strictness with which probes and targets hybridize. The higher the stringency is set, the more complimentary the probe and target must be to hybridize.