Configure an AmpliSeq for Illumina Hotspot Design
DNA from high-quality FFPE tissue can be used with longer amplicon designs. However, uniformity and representation of longer amplicons can decrease.
||Select the Max Amplicon Length.
375 bp is recommended for MiSeq only.
||[Optional] Select Learn more about stringency for information on how different stringency levels impact the final assay design.
||Select a Stringency from the drop-down list.
Stringency represents the strictness with which probes and targets hybridize. The higher the stringency is set, the more complimentary the probe and target must be to hybridize.