HiSeq 4000 Documentation

  • Supporting Documents

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  • Read 1 Primer Rehybridization on a HiSeq 3000/4000 Flow Cell

    Instructions for performing Read 1 primer rehybridization on a HiSeq 3000/4000 flow cell using the HiSeq 3000/4000 cBot Multi-Primer Rehybridization Kit.

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    Feb 8, 2016


  • Cluster Optimization Overview

    Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

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    Apr 26, 2021


  • Indexed Sequencing Overview

    The sequencing process for single- and dual-indexed runs on Illumina instruments.

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    Aug 13, 2024


  • Security and Networking

    Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

    It is strongly recommended that you review this documentation to keep your instrument secure.

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    Jan 12, 2024