HiSeq 3000 Reagent Kits

Catalog IDs: PE-410-1001, GD-410-1001, FC-410-1001, FC-410-1002, FC-410-1003

Kit Requirements

Performing a run requires one HiSeq 3000/4000 Cluster Kit and one HiSeq 3000/4000 SBS Kit.

  • The cluster kit is available in paired-end (PE) and single-read (SR) versions. Both versions include clustering reagents for the cBot 2 or cBot System and indexing and paired-end reagents for the HiSeq 3000 System.
  • The SBS kit is available in three sizes: 300-, 150-, and 50-cycle. All sizes include sequencing reagents to sequence one flow cell on the HiSeq 3000 System.
  • Both kits are single-use.

For other system requirements and compatible products, see HiSeq 3000 Compatibility & Requirements.

Contents and Storage

HiSeq 3000/4000 Cluster Kit

The HiSeq 3000/4000 Cluster Kit includes a patterned flow cell, a cBot manifold, two boxes, and an accessory kit. The flow cell format depends on the kit:

  • The PE kit includes a PE flow cell. Store at 2°C to 8°C.
  • The SR kit includes an SR flow cell. Store at 2°C to 8°C.
Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Reagent Plate

PE Kit—cBot reagent plate, PE

SR Kit—cBot reagent plate, SR

1

RSB

Resuspension Buffer

1

EPX1

Enhanced Pattern Cluster Mix 1

1

EPX2

Enhanced Pattern Cluster Mix 2

1

EPX3

Enhanced Pattern Cluster Mix 3

PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HRM

HT Resynthesis Mix

1

HLM2

HT Linearization Mix 2

1

HAM

HT Amplification Mix

1

HPM

HT Amp Premix

1

HDR

HT Denaturation Mix (contains formamide)

1

HP11

Primer Mix – Read 2

1

HP14

Indexing Primer Mix

SR Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HRM

HT Resynthesis Mix

1

HDR

HT Denaturation Mix
(contains formamide)

1

HP12

Index 1 Primer Mix

Accessory Kit

Quantity

Accessory

Purpose

1

Flow cell gaskets (package of 4)

Replace the flow cell gaskets between runs.

1

Flow cell storage tube with storage buffer, 50 ml

Store the flow cell between clustering and sequencing.

1

Funnel bottle caps (package of 8)

Replace the caps on SBS reagent bottles before loading reagents.

HiSeq 3000/4000 SBS Kit
SBS Kit Box 1

Kit Component

Storage

Description

PW1

15°C to 30°C Wash Buffer
HB1 15°C to 30°C High Throughput SBS Buffer 1
HB2 15°C to 30°C High Throughput SBS Buffer 2
SBS Kit Box 2

Kit Component

Storage

Description

HCM

-25°C to -15°C

High Throughput Cleavage Mix

HIM

-25°C to -15°C High Throughput Incorporation Mix
HSM -25°C to -15°C High Throughput Scan Mix