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Target Hits File Format
The TargetHitsPerSample_M1.tsv file is a tab-delimited file that contains the raw aligned replicate counts for each transcript.
The header of the file contains one row of column headings including sample IDs, followed by a row of sample names. For differential analysis, there must be at least two distinct sample names in the sample sheet.
This table describes the fields in the Target Hits file.
Column Heading |
Description |
Gene Name |
The name of the gene. |
Amplicon ID |
The amplicon identifier constructed from the gene name, transcript ID, left exon, right exon, and assay ID. |
Assay ID |
The unique identifier for the probe set. |
Sample ID |
Aligned count for all transcripts for this sample. There is one column for each sample using this manifest. |