Requirements & Compatibility

• Version Compatibility of Run Components
• Sequencing Primers for Dual-Indexed Libraries
• SBS Reagents
• Indexing Reagents
• Paired-End Reagents
• Sequencing Accessories
• Product Compatibility

Version Compatibility of Run Components

For best performance and run results, always use compatible versions of SBS reagents and HiSeq Control Software for the flow cell you are using.

Sequencing Primers for Dual-Indexed Libraries

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2000:

• Any Nextera libraries with any number of indices for single-read or paired-end runs. Use the single-read kit with single-read flow cells, and the paired-end kit with paired-end flow cells.
• TruSeq HT libraries when performing a dual-indexed run on a single-read flow cell.

Sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box,  HP10, HP11, and HP12, replace reagents provided in the TruSeq Cluster Kit. Use HP10 instead of HP6 on the cBot. Use HP11 instead of HP7, and HP12 instead of HP8 on the HiSeq. For more information, see the HiSeq 2000 User Guide.

SBS Reagents

One TruSeq SBS Kit is required for sequencing one flow cell on the HiSeq. One 200-cycle kit performs up to 209 cycles of sequencing; one 50-cycle kit performs up to 58 cycles of sequencing.

Indexing Reagents

Indexing reagents are used for the Index Read of an indexed sequencing run. One box of indexing reagents, provided in the TruSeq Cluster Kit (PE or SR), is required for sequencing one flow cell.

Paired-End Reagents

Paired-end reagents are used for the cluster resynthesis step in a paired-end sequencing run. One box of paired-end reagents, provided in the TruSeq PE Cluster Kit, is required for sequencing one paired-end flow cell.

Sequencing Accessories

The TruSeq Cluster Kit (cBot - HS) contains an accessories kit, which includes components used for setting up a sequencing run on the HiSeq 2000.