TruSeq Small RNA Sample Prep Kit

Catalog IDs: RS-200-0012, RS-200-0024, RS-200-0036, RS-200-0048

Features

  • Enables preparation of a variety of RNA species, using total RNA or purified small RNA as input.
  • Takes advantage of the natural structure common to most known microRNA molecules.
  • Protocol includes adapter ligation, reverse transcription, PCR amplification, and pooled gel purification to generate a library product.
  • RNA 3' adapter is specifically modified to target microRNAs and other small RNAs that have a 3' hydroxyl group resulting from enzymatic cleavage by Dicer or other RNA processing enzymes.
  • Adapters are ligated to each end of the RNA molecule and an RT reaction is used to create single-stranded cDNA.
  • cDNA is PCR amplified using a common primer and a primer containing one of 48 index sequences.
  • Introduction of the index sequence at the PCR step separates the indexes from the RNA ligation reaction, allowing for the indexes to be read using a second read and significantly reducing bias compared to designs which include the index within the first read.
  • Allows the use of 48 different index tags to make use of the Illumina multiplexing capability for analyzing directional and small RNA samples.
  • Employs six-base indices to distinguish different samples from one another in a single lane of a flow cell.

TruSeq RNA Sample Prep Kit v2