The PhiX Control v3 Library (commonly referred to as PhiX, FC-110-3001) is derived from the small, well characterized bacteriophage PhiX genome. It is a concentrated Illumina library (10 nM in 10 µl) that has an average size of 500 bp and consists of balanced base composition at ~45% GC and ~55% AT.
A PhiX Control v3 Library can be run alone and serves as a calibration control for:
For detailed information, see:
Run Quality Monitor
Due to its small size and balanced nucleotide composition, PhiX Control v3 Library is also an ideal in-run control (typically with ≥ 1% spike-in) for run quality monitoring, eg, cluster generation, sequencing, and alignment.
For low diversity libraries, the PhiX Control v3 Library provides balanced fluorescent signals at each cycle to improve the overall run quality. For detailed information, see: