What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing?


The PhiX Control v3 Library (commonly referred to as PhiX, FC-110-3001) is derived from the small, well characterized bacteriophage PhiX genome. It is a concentrated Illumina library (10 nM in 10 µl) that has an average size of 500 bp and consists of balanced base composition at ~45% GC and ~55% AT.

Calibration Control

A PhiX Control v3 Library can be run alone and serves as a calibration control for:

  • Cross talk matrix generation
  • Phasing and prephasing calculations
  • Examine the overall performance of Illumina sequencing platforms

For detailed information, see:

Run Quality Monitor

Due to its small size and balanced nucleotide composition, PhiX Control v3 Library is also an ideal in-run control (typically with ≥ 1% spike-in) for run quality monitoring, eg, cluster generation, sequencing, and alignment.

Color Balancing

For low diversity libraries, the PhiX Control v3 Library provides balanced fluorescent signals at each cycle to improve the overall run quality. For detailed information, see:

What is nucleotide diversity and why is it important?