Summary Analysis Report
The RNA-Seq Alignment App provides an aggregate summary for all the samples.
|
Statistic |
Definition |
|---|---|
|
Sample ID |
The sample ID. |
|
Number of Reads |
The total number of reads passing filter for this sample. |
|
Read Length |
The average length of input reads in basepairs. |
|
% Abundant |
Percentage of reads that match abundant sequences, such as mitochondrial and ribosomal sequences. These fragments are filtered. |
|
% Aligned |
Percentage of reads passing filter that aligned to the reference. |
|
Median CV Coverage Uniformity |
The median coefficient of variation of coverage of the 1000 most highly expressed transcript, as reported by the CollectRnaSeqMetrics utility from Picard tools. Ideal value = 0. |
|
% Stranded |
Percentage of reads that align to the expected strand of annotated transcripts. |