Alignment Metrics

Statistic

Definition

Total Number of Reads

Total number of reads passing filter for this sample.

Read Length (bp)

Length of reads

Abundant Reads (% Total)

Percentage of reads that match to abundant sequences, such as mitochondrial and ribosomal sequences.

Total Number of Filtered Reads

Total number of reads filtered out at the abundant sequence filtering step.

Aligned Reads (% Passing Reads)

Percentage of reads passing abundant sequence filters that aligned to the reference.

Reads with Spliced Alignment (% Aligned Reads)

Percentage of aligned reads with a spliced alignment.

Reads Aligned at Multiple Loci (% Aligned Reads)

Percentage of aligned reads to multiple loci.