Coverage Summary
We exclude duplicate reads from the coverage statistics. We calculate the target coverage percentage for non-N reference positions in the targeted regions. Overlapping reads (read1/read2 in a pair) count as
|
Statistic |
Definition |
|---|---|
|
Mean Target Coverage Depth |
The total number of targeted bases divided by the targeted region size. |
|
Uniformity of Coverage (Pct > 0.2*mean) |
The percentage of targeted base positions in which the read depth is greater than 0.2 times the mean region target coverage depth. |
|
Percent Target Unique Overlapping Bases |
The percent of total PF bases where the base call corresponds to a position on a fragment of a paired-end read that is called in Read 1 and Read 2. |
|
Target Coverage at 1X |
Percentage targets with coverage greater than 1X. |
|
Target Coverage at 10X |
Percentage targets with coverage greater than 10X. |
|
Target Coverage at 20X |
Percentage targets with coverage greater than 20X. |
|
Target Coverage at 50X |
Percentage targets with coverage greater than 50X. |