Enrichment Summary
Unless it is requested, all enrichment values are calculated without padding. When a targeted region overlaps another targeted region, the region positions are adjusted to remove overlaps. You can check the manifest file for differences. The total length of the targeted reference excludes no-call positions (Ns) in the reference sequence. As a result, the total length can have a smaller value from the targeted regions coordinates.
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Statistic |
Description |
|---|---|
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Target Manifest |
The target manifest name. |
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Total Length of Targeted Reference |
The total length of the sequenced bases in the target region. |
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Number of Targeted Regions |
The number of targeted regions in the input manifest. |
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Padding Size |
The length of sequence immediately upstream and downstream of the enrichment targets that is included for a padded target. |
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Statistic |
Description |
|---|---|
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Total Aligned Reads |
The total number of reads passing filter present in the data set that aligned to the reference genome. |
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Percent Aligned Reads |
The percentage of reads passing filter that aligned to the reference genome. |
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Target Aligned Reads |
Number of reads that aligned to the target. |
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Read Enrichment |
100*(Target aligned reads/Total aligned reads). |
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Padded Target Aligned Reads |
Number of reads that aligned to the padded target. |
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Padded Read Enrichment |
100*(Padded target aligned reads/Total aligned reads). |
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Statistic |
Description |
|---|---|
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Total Aligned Bases |
The total number of bases present in the data set that aligned to the reference genome. |
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Target Aligned Bases |
Total aligned bases in the target region. |
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Bases Enrichment |
100*(Total Aligned Bases in Targeted Regions/Total Aligned Bases). |
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Padded Target Aligned Bases |
Total aligned bases in the padded target region. |
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Padded Base Enrichment |
100*(Total Aligned Bases in Padded Targeted Regions/Total Aligned Bases). |
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Statistic |
Description |
|---|---|
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Number of Targeted Regions Covered End-to-End |
The number of targeted regions that have at least 1X coverage across all on-N reference positions. |
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Percentage of Targeted Regions Covered End-to-End |
The percent of targeted regions that have a least 1X coverage across all non-N reference positions. |