Isaac Aligner
The Isaac Aligner software uses the following steps to align DNA sequencing data with read lengths of 36–150 bp, single or paired-end, and low error rates.
| • | Candidate mapping positions—Identifies the complete set of relevant candidate mapping positions using a 32-mer seed-based search. |
| • | Mapping selection—Selects the best mapping among all candidates. |
| • | Alignment score—Determines alignment scores for the selected candidates based on a Bayesian model. |
| • | Alignment output—Generates final output in a sorted and duplicate-marked BAM file, realigned indels, and a summary file. |