NextSeq 1000/2000 Sequencing System Documentation

  • Online Help

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  • NextSeq 1000/2000 Product Documentation v01

    Instructions for operating and maintaining the NextSeq 1000/2000 Instrument (English only).

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    May 31, 2024


  • Supporting Documents

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  • Indexed Sequencing Overview

    The sequencing process for single- and dual-indexed runs on Illumina instruments.

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    Apr 26, 2021


  • Security and Networking

    Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

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    Jan 12, 2024


Product Literature

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Data concordance between the NextSeq 1000/2000 and NextSeq 550 Sequencing Systems
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Application note < 1 MB Mar 23, 2020
Illumina Sequencing Platforms Brochure
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Brochure 15 MB
DRAGEN Bio-IT Platform Onboard NextSeq 1000 and NextSeq 2000 Systems
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Data sheet < 1 MB Aug 2, 2022
NextSeq 1000 and NextSeq 2000 Sequencing Systems specification sheet
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Data sheet < 1 MB May 30, 2024
Explore the transcriptome with single-cell resolution
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Technical note 1 MB Mar 1, 2022
Data Security with Illumina Proactive
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Technical note < 1 MB Oct 4, 2022
Multiomic interrogation of the immune system at single-cell resolution
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Technical note 1 MB Mar 1, 2022
Unify single-cell gene expression and chromatin accessibility
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Technical note 1 MB
Resolve the whole transcriptome within tissue architecture
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Technical note 3 MB Jul 1, 2021
XLEAP-SBS chemistry expands NextSeq 1000 and NextSeq 2000 System capabilities
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Technical note < 1 MB
Full-length V(D)J immune repertoire sequencing (IR-Seq) on the NextSeq™ 1000 and NextSeq 2000 Systems
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Application note < 1 MB
16S rRNA sequencing on NextSeq™ 1000 and NextSeq 2000 Systems
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Application note < 1 MB Mar 16, 2023
High-plex spatial proteogenomics of FFPE tissue sections
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Application note 2 MB
NextSeq™ 1000 and NextSeq 2000 single-cell RNA sequencing solution
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Application note < 1 MB May 30, 2024
NextSeq 1000 and NextSeq 2000 RNA sequencing solution
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Application note < 1 MB May 30, 2024
A shotgun metagenomics NGS workflow for assessing microbial populations in complex samples
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Application note < 1 MB Mar 13, 2024
High-resolution, high-throughput spatial transcriptomics of complex tissues
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Application note 3 MB Aug 24, 2021
Small whole-genome sequencing on NextSeq 1000 and NextSeq 2000 Systems
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Application note < 1 MB
NextSeq 1000 and NextSeq 2000 exome sequencing solution
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Application note < 1 MB