NextSeq 500 System

Current Consumables

NextSeq 500 High Output Kit—Available in three sizes (300 cycles, 150 cycles, and 75 cycles), the NextSeq 500 High Output Kit includes a high-output flow cell, a high-output reagent cartridge, and a buffer cartridge.

NextSeq 500 Mid Output Kit—Available in two sizes (300 cycles and 150 cycles), the NextSeq 500 Mid Output Kit includes a mid-output flow cell, a mid-output reagent cartridge, and a buffer cartridge.

Kit Versions and Compatibility—The NextSeq 500 Kit v2 requires NCS v1.4. If using the original NextSeq 500 Kit (v1), continue using NCS v1.3.

Current Software

NextSeq Control Software—The control software interface guides you through the steps to load the flow cell and reagents before beginning a sequencing run. During the run, the NextSeq Control Software (NCS) operates the flow cell stage, dispenses reagents, controls flow cell temperatures, and captures images of clusters on the flow cell.

NCS v1.4—Required for use with the NextSeq 500 Kit v2. NCS v1.4 is optimized for v2 reagents. Earlier kit versions are not compatible.

NCS v1.3—Compatible NextSeq v1 reagents.

Real-Time Analysis—The NextSeq 500 uses an updated implementation of Real-Time Analysis (RTA) called RTA v2, which includes important architecture differences from RTA used on other Illumina sequencers. Unlike earlier versions of RTA, all processes are performed in memory to maximize processing speed. RTA is part of the NextSeq system software.

Analysis Solutions

The NextSeq 500 is enabled to integrate with BaseSpace, providing an end to end data storage, sharing, and analysis system. The NextSeq 500 can seamlessly stream data to BaseSpace, where demultiplexing and conversion to industry-standard FASTQ file formats occurs automatically as the final step in data transfer to Basespace.

Use the bcl2fastq 2.0 conversion software to convert NextSeq 500 output to standard FASTQ file formats for downstream analysis using third-party analysis solutions. Version 2.0 is only for use with NextSeq data. For more information, see the bcl2fastq v2.0 Conversion User Guide.

Illumina NextSeq 500