NovaSeq 6000 Documentation

  • Supporting Documents

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  • Cluster Optimization Overview

    Strategies for optimizing cluster density and preventing and diagnosing clustering issues on Illumina flow cells.

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    Apr 26, 2021


  • Sequencing Human Whole Genomes on the NovaSeq 6000 System

    Information and recommendations for performing Human Whole-Genome Sequencing (WGS) on the NovaSeq 6000 Sequencing System.

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  • Indexed Sequencing Overview

    The sequencing process for single- and dual-indexed runs on Illumina instruments.

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    Apr 26, 2021


  • Security and Networking

    Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

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    Jan 12, 2024


Product Literature

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The NovaSeq 6000 System Specification Sheet
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Data sheet
Human Whole-Genome Sequencing with the NovaSeq 6000 Sequencing System
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Application note < 1 MB Aug 31, 2018
NovaSeq 6000 System Quality Scores and RTA3 Software
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Application note < 1 MB Oct 19, 2017
Sequencing Library QC on the iSeq System
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Application note < 1 MB Nov 2, 2018
The NovaSeq Xp workflow provides flexibility and control without sacrificing data quality or yield
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Technical note < 1 MB Jan 15, 2019
An automated Nextera DNA Flex library preparation workflow for high-throughput metagenomics
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Application note < 1 MB May 16, 2019
QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 System
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Application note 2 MB Jan 29, 2020
High-performance whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation
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Application note < 1 MB Jun 9, 2020
De Novo Genome Assembly with Illumina Sequencing and the NRGene DeNovoMAGIC Solution
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Application note
NovaSeq 6000 Sequencing System
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Data sheet < 1 MB Jul 26, 2022
NovaSeq 6000 Sequencing System
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Data sheet < 1 MB Feb 7, 2022
Explore the transcriptome with single-cell resolution
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Technical note 1 MB Mar 1, 2022
Data Security with Illumina Proactive
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Technical note < 1 MB Oct 4, 2022
Impact of NovaSeq flow cell lane edge integrity on sequencing performance
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Technical note 1 MB Dec 15, 2021
Population genetics data processing with the DRAGEN Bio-IT Platform
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Technical note 1 MB
Multiomic interrogation of the immune system at single-cell resolution
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Technical note 1 MB Mar 1, 2022
Sequencing performance with NovaSeq Control Software v1.8
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Technical note < 1 MB
Unify single-cell gene expression and chromatin accessibility
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Technical note 1 MB
Resolve the whole transcriptome within tissue architecture
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Technical note 3 MB Jul 1, 2021
Enhanced sequencing capabilities with the NovaSeq™ 6000 v1.5 Reagent Kit
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Technical note < 1 MB Sep 30, 2020
Balancing sample coverage for whole-genome sequencing
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Technical note < 1 MB Apr 5, 2021
Long read lengths for shotgun metagenomics
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Application note < 1 MB Aug 12, 2021
Illumina Genomics Architecture enables PopGen studies with Illumina DNA PCR-Free Prep
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Application note 2 MB Sep 7, 2023
High-plex spatial proteogenomics of FFPE tissue sections
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Application note 2 MB
Ultra-long range phasing with linked-read sequencing technology
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Application note 1 MB
High-resolution, high-throughput spatial transcriptomics of complex tissues
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Application note 3 MB Aug 24, 2021