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Target Hits File Format
The TargetHitsPerSample_M1.tsv file is a tab-delimited file that contains the raw aligned replicate counts for each transcript.
The header of the file contains one row of column headings including sample IDs, followed by a row of sample names. For differential analysis, there must be at least two distinct sample names in the sample sheet.
This table describes the fields in the Target Hits file.
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Column Heading |
Description |
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Gene Name |
The name of the gene. |
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Amplicon ID |
The amplicon identifier constructed from the gene name, transcript ID, left exon, right exon, and assay ID. |
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Assay ID |
The unique identifier for the probe set. |
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Sample ID |
Aligned count for all transcripts for this sample. There is one column for each sample using this manifest. |