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RNA Sample Metrics Report
File name: RNA_SampleMetricsReport.txt
Review this report to assess RNA sample data quality by evaluating the following metrics.
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Metric |
Description |
Output File Evaluated |
Quality Threshold |
|---|---|---|---|
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Median_Insert_Size |
The median of fragment sizes calculated from read alignments |
Fusions.csv, HighConfidenceVariants.csv, SpliceVariants.vcf, PublishedFusions.csv |
≥1 |
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Median_CV_Coverage_1000x |
The median of the CV value of target region coverage across all genes with coverage above 1000 |
Fusions.csv, HighConfidenceVariants.csv, SpliceVariants.vcf, PublishedFusions.csv |
≤0.88 |
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PCT_Chimeric_Reads |
Percentage of reads that are part of a chimeric alignment (ie, one half of the read maps to one location and the other half maps to a nonsequential position) out of total mapped reads |
Not used to evaluate sample data quality |
N/A |
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PCT_On_Target_Reads |
Percentage of reads that map to target regions out of total PF reads |
Not used to evaluate sample data quality |
N/A |
For Research Use Only. Not for use in diagnostic procedures.