Analysis Methods/DNA Analysis/Determining Microsatellite Instability

Determining Microsatellite Instability

Microsatellite instability (MSI) is determined from the BAM file, and then an MSI metric file is created. Using information entropy metrics, the software assesses microsatellite sites for evidence of instability relative to a set of baseline normal samples. The proportion of unstable MSI sites to total assessed MSI sites is reported as a sample-level microsatellite score.