Set Analysis Parameters
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1.
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Launch the app from BaseSpace Sequence Hub. |
- Select the Apps tab.
- Select TruSight Tumor 170.
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From the drop-down list, select version 2.0.0, and then select Launch Application.
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2.
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[Optional] In the Analysis Name field, enter an analysis name of your preference. |
The default is the app name followed by the date and time the analysis session started.
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3.
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Select the Select Project button. |
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4.
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In the Select Project dialog box, do one of the following: |
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Select an existing project to store the app results. |
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Select New to create a project. Add a name and description, and select Create to add it to the projects list. |
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5.
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Select Select in the Select Project dialog box. |
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6.
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For DNA Intermediate Files, select which BAM files to keep from the following options: |
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Keep aligned and realigned BAM files.
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Keep aligned BAM files only.
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Keep realigned BAM files only.
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Do not keep any BAM files.
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The analysis generates a set of BAM files after alignment and a second set of BAM files after indel realignment. Deleting BAM files may reduce long‑term data storage costs. It is not possible to delete individual BAM files from the app results.
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7.
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Select Select Pairs to select samples for a pair. |
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8.
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Select samples to analyze, and drag them to either the DNA or RNA column. Select Filters to filter the Samples list by project. |
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Pairs must have equivalent read lengths. |
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The maximum number of samples is 16. |
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DNA and RNA samples can be analyzed individually. |
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9.
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When sample selection is complete, select Confirm. |
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10.
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Select Launch Application. |
When the analysis completes, the app session status automatically updates.