Workflow Requirements
| • | Supports Illumina® TruSight Tumor 170 assay |
| • | Compatible with NextSeq or HiSeq 2500 Rapid Run systems |
| • | Sequencing settings: |
| • | 2 x 101 (recommended) |
| • | Paired End (required) |
| • | Connectivity to send sequencing run output data to BaseSpace Sequence Hub |
| • | Additional specifications can be found in the TruSight Tumor 170 data sheet |
| • | Sample IDs on sample sheets configured using the Sample Sheet Template or the BaseSpace® Sequence Hub Prep Tab must meet the following criteria: |
| • | < 60 characters |
| • | Alphanumeric, underscore, and dash characters only |
| • | No duplicate Sample ID |
| • | Alphanumeric characters must precede and follow any underscores and dashes |
| • | Sample names cannot begin or end with an underscore or dash |
| • | Sequencing Run Setup |
| • | Optional for NextSeq, Import CSV template for BaseSpace® Sequence Hub Prep Tab |
| • | Required for HiSeq 2500 Rapid Run, Sample Sheet Template |
| • | For DNA analysis |
| • | Minimum of |
| • | Paired-end samples with the same read lengths are required |
| • | For RNA analysis |
| • | Minimum of 16 M reads is recommended |
| • | Paired-end samples with the same read lengths are required |