Analysis Output | Sample Analysis Reports | Amplicon Summary

Amplicon Summary

Table 4 Amplicon Summary Table
Statistic	Definition
Number of Amplicons	The number of amplicon regions that were sequenced.
Total Length of Amplicon	The total length of the sequenced amplicon regions in base pairs.

Table 5 Read Level Statistics Table
Statistic	Definition
Total Aligned Reads	The total number of reads passing filter present in the data set that aligned to the reference genome.
Percent Aligned Reads	The percentage of reads passing filter that aligned to the reference genome.

Table 6 Base Level Statistics Table
Statistic	Definition
Total Aligned Bases	The total number of bases present in the data set that aligned to the reference genome.
Percent Aligned Bases	The percentage of bases that aligned to the reference genome.
Percent Q30	The percentage of bases with a quality score of 30 or higher.
Mismatch Rate	The average percentage of mismatches across both reads 1 and 2 over all cycles.