Isaac Aligner
The Isaac software uses the following steps to align DNA sequencing data with read lengths of 32–150 bp, single or paired-end, and low error rates.
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Candidate mapping positions—Identifies the complete set of relevant candidate mapping positions using a 32-mer seed-based search. |
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Mapping selection—Selects the best mapping among all candidates. |
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Alignment score—Determines alignment scores for the selected candidates based on a Bayesian model. |
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Alignment output—Generates final output in a sorted and duplicate-marked BAM file and summary file. |
Whole Genome Sequencing v5.0 App Online Help
For Research Use Only. Not for use in diagnostic procedures.