我所购买的SBS试剂盒最多能够满足多少个循环的测序？

为上机测序提供足够量的SBS试剂是确保测序获得成功的重要因素之一。如果运行过程中试剂量不够，会导致荧光信号强度和测序质量值降低，甚至可能引起测序失败。双端测序在不同测序仪上有不同的操作流程，具体可以参照Indexed Sequencing Overview Guide。在某些仪器上运行双端index测序时，还需要额外七个循环的试剂做第二个index测序前的化学合成反应（仅合成不拍照），此时计算试剂盒所能支持的测序循环数时，一定要考虑到这七个循环也要被包含在总循环数内。

下面表格列举出了多种试剂盒的规格及每种试剂盒最多支持的循环数，请将在某些仪器上运行双端index测序时额外的7个循环考虑在内:

Reagent Type		Reagent Kit Size	Maximum Number of Cycles	Additional Cycles Needed for Dual Index?
iSeq 100	i1 (v1 or v2)	300	322	No
MiniSeq	Rapid Kit	100	128	Yes - 7 cycles
	High Output or Mid Output	75	92	No
		150	168
		300	318
MiSeq	v2 (including Micro and Nano kits)	50	79	Yes - 7 cycles
		300	329
		500	529
	v3	150	179
	v3	600	629
NextSeq 500/550	High Output or Mid Output	75	92	No
		150	168
		300	318
HiSeq 1000/1500/2000/2500	Rapid SBS v2	50	79	7 cycles required for paired-end flow cells
		200	229
		500	529
	TruSeq SBS v3	50	58
	TruSeq SBS v3	200	209
	HiSeq SBS v4	50	79
	HiSeq SBS v4	250	279
NextSeq 1000/2000
	P1	100	138	No
		300	338
		600	638
	P2	100	138	No
		200	238
		300	338*
		600	638
	P3	50	88	No
		100	138
		200	238
		300	327*
HiSeq 3000/4000	N/A	50	79	7 cycles required for single-read flow cells
		150	179
		300	329
NovaSeq 6000	SP v1.0	100	130	Yes - 7 cycles
		200	230
		300	330
		500	530
	S1 and S2 v1.0	100	130
		200	230
		300	330
	S4 v1.0	200	230
	S4 v1.0	300	330
	SP v1.5	100	138	No
		200	238
		300	338
		500	538
	S1 and S2 v1.5	100	138
		200	238
		300	338
	S4 v1.5	35	72
		200	238
		300	338

*The NextSeq 1000/2000 P1和P2试剂盒含有338个cycles 而 P3 v3 试剂盒含有327个cycles.

关于每种测序试剂盒的详细信息请参阅对应的试剂支持页面，不同测序试剂盒支持的最长读长请参阅Maximum read length for Illumina sequencing platforms

我所购买的SBS试剂盒最多能够满足多少个循环的测序？

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我所购买的SBS试剂盒最多能够满足多少个循环的测序？

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