This bulletin outlines the features and specifications of the new P1 reagent kit for the NextSeq 1000 and NextSeq 2000 systems.
What’s new:
- Usage of the new NextSeq 1000/2000 P1 reagent kit is enabled by an upgrade to NextSeq 1000/2000 Control Software Suite v1.4. This upgrade is required to run the new kit and can be self-installed.
- The P1 reagent kit introduces a lower throughput option for NextSeq 1000/2000 users.
- Along with the previously available P2 and P3 kits, the P1 kit expands the scalability and flexibility of the NextSeq 1000/2000 for more applications while allowing smaller batches, and projects to be run in < 20 hours.
Table 1: NextSeq 1000/2000 P1 specifications compared to P2 and P3 kits.
|
Read length
|
NextSeq 1000/2000
P1
|
NextSeq 1000/2000
P2
|
NextSeq 2000
P3
|
|
Output per flow cell
|
|
1 x 50 bp (P3 only)
|
N/A |
N/A |
60 Gb
|
|
2 x 50 bp
|
N/A |
40 Gb
|
120 Gb
|
|
2 x 100 bp
|
N/A |
80 Gb
|
240 Gb
|
|
2 x 150 bp
|
30 Gb
|
120 Gb
|
360 Gb
|
|
Reads Passing Filter
|
100M
|
400M
|
1.2B
|
|
Quality scores
|
|
1 x 50 bp
|
≥ 90% of bases higher that Q30
|
|
2 x 50 bp
|
≥ 90% of bases higher that Q30
|
|
2 x 100 bp
|
≥ 85% of bases higher that Q30
|
|
2 x 150 bp
|
≥ 85% of bases higher that Q30
|
|
Run time
|
|
1 x 50 bp
|
N/A |
N/A |
~11 hours
|
|
2 x 50 bp
|
N/A |
~13 hours
|
~19 hours
|
|
2 x 100 bp
|
N/A |
~21 hours
|
~33 hours
|
|
2 x 150 bp
|
~19 hours
|
~29 hours
|
~48 hours
|
Frequently asked questions:
- What sizes of P1 reagents are available?
The NextSeq 1000/2000 P1 kit is only available in a 300-cycle size.
- What NextSeq 1000/2000 Control Software version is required to run the P1 kit?
Version 1.4 of the NextSeq 1000/2000 Control Software introduces compatibility with the P1 kit; older versions of the control software are not compatible.
- Are the data from the P1 kit compatible with the onboard DRAGEN workflows?
Yes, with NextSeq 1000/2000 Control Software v1.4, onboard DRAGEN versions 3.7 and 3.8 are both compatible with P1 data.
- What is the expected run time for the P1 kit?
A full 2 x 150 bp paired-end run takes around 19 hours to complete, with an average cycle time of about 2.8 minutes.
- Are there any changes in loading concentration compared to other NextSeq 1000/2000 kits?
The P1 kit uses the same loading concentration as the P2 and P3 kits.
- How many cycles are in the P1 300-cycle kit?
The P1 300-cycle reagent cartridge includes sufficient volume for an extra 38 cycles, allowing up to 338 cycles of sequencing.