Contents & Storage

 

TruSeq Stranded Total RNA Library Prep (48 Samples)

Library Prep Box 1, Store as specified

Quantity

Reagent

Description

Storage

1 DTE CTE Dilution Tube Room Remperature
1 DTA CTA Dilution Tube Room Remperature
1 DTL CTL Dilution Tube Room Remperature
1 RRB rRNA Removal Beads 2°C to 8°C

Library Prep Ribo-Zero Box, Store as Specified

You receive one of the following boxes, depending on the workflow. The box also contains plate barcode labels.

Ribo-Zero H/M/R

Quantity

Reagent

Description

Storage

1 RBB rRNA Binding Buffer -25°C to -15°C
1 RRM rRNA Removal Mix -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Ribo-Zero Gold

Quantity

Reagent

Description

Storage

1 RBB rRNA Binding Buffer -25°C to -15°C
1 RRM G rRNA Removal Mix - Gold -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Ribo-Zero Plant

Quantity

Reagent

Description

Storage

1 RBB rRNA Binding Buffer -25°C to -15°C
1 RRM P rRNA Removal Mix - Plant -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Ribo-Zero Globin

Quantity

Reagent

Description

Storage

1 RBB rRNA Binding Buffer -25°C to -15°C
1 GRM Globin Removal Mix -25°C to -15°C
1 ELB Elution Buffer 2°C to 8°C
1 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Core Library Prep Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

 

1 ATL A-Tailing Mix  
1 CTA A-Tailing Control  
1 CTE End Repair Control  
1 CTL Ligation Control  
1 LIG Ligation Mix  
1 RSB Resuspension Buffer  
1 STL Stop Ligation Buffer  

Core Library Prep Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

 

1 PMM PCR Master Mix  
1 PPC PCR Primer Cocktail  
1 FSA First Strand Synthesis Act D Mix  
1 SMM Second Strand Marking Master Mix  

TruSeq Stranded Total RNA Library Prep (96 Samples)

A quantity of two of each box is included for the 96 sample workflow.

Library Prep Box 1, Store as specified

Quantity

Reagent

Description

Storage

1 DTE CTE Dilution Tube Room Remperature
1 DTA CTA Dilution Tube Room Remperature
1 DTL CTL Dilution Tube Room Remperature
2 RRB rRNA Removal Beads 2°C to 8°C

Library Prep Ribo-Zero Box, Store as Specified

You receive two of one of the following boxes, depending on the workflow. The box also contains plate barcode labels.

Library Preparation H/M/R (48 Samples)

Quantity

Reagent

Description

Storage

2 RBB rRNA Binding Buffer -25°C to -15°C
2 RRM rRNA Removal Mix -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Library Preparation Gold (48 Samples)

Quantity

Reagent

Description

Storage

2 RBB rRNA Binding Buffer -25°C to -15°C
2 RRM G rRNA Removal Mix - Gold -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Library Preparation Plant (48 Samples)

Quantity

Reagent

Description

Storage

2 RBB rRNA Binding Buffer -25°C to -15°C
2 RRM P rRNA Removal Mix - Plant -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Library Preparation Globin (48 Samples)

Quantity

Reagent

Description

Storage

2 RBB rRNA Binding Buffer -25°C to -15°C
2 GRM Globin Removal Mix -25°C to -15°C
2 ELB Elution Buffer 2°C to 8°C
2 EPH Elute, Prime, Fragment High Mix -25°C to -15°C

Core Library Prep Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

 

2 ATL A-Tailing Mix  
2 CTA A-Tailing Control  
2 CTE End Repair Control  
2 CTL Ligation Control  
2 LIG Ligation Mix  
2 RSB Resuspension Buffer  
2 STL Stop Ligation Buffer  

Core Library Prep Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

 

2 PMM PCR Master Mix  
2 PPC PCR Primer Cocktail  
2 FSA First Strand Synthesis Act D Mix  
2 SMM Second Strand Marking Master Mix  

For information about the Inline Control DNA provided in this kit, see the Inline Control DNA section of the the TruSeq Stranded Total RNA Reference Guide.