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Contents & Storage

TruSeq Stranded Total RNA Library Prep (48 Samples)

Library Prep Box 1, Store as specified

Quantity	Reagent	Description	Storage
1	DTE	CTE Dilution Tube	Room Remperature
1	DTA	CTA Dilution Tube	Room Remperature
1	DTL	CTL Dilution Tube	Room Remperature
1	RRB	rRNA Removal Beads	2°C to 8°C

Library Prep Ribo-Zero Box, Store as Specified

You receive one of the following boxes, depending on the workflow. The box also contains plate barcode labels.

Ribo-Zero H/M/R

Quantity	Reagent	Description	Storage
1	RBB	rRNA Binding Buffer	-25°C to -15°C
1	RRM	rRNA Removal Mix	-25°C to -15°C
1	ELB	Elution Buffer	2°C to 8°C
1	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Ribo-Zero Gold

Quantity	Reagent	Description	Storage
1	RBB	rRNA Binding Buffer	-25°C to -15°C
1	RRM G	rRNA Removal Mix - Gold	-25°C to -15°C
1	ELB	Elution Buffer	2°C to 8°C
1	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Ribo-Zero Plant

Quantity	Reagent	Description	Storage
1	RBB	rRNA Binding Buffer	-25°C to -15°C
1	RRM P	rRNA Removal Mix - Plant	-25°C to -15°C
1	ELB	Elution Buffer	2°C to 8°C
1	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Ribo-Zero Globin

Quantity	Reagent	Description	Storage
1	RBB	rRNA Binding Buffer	-25°C to -15°C
1	GRM	Globin Removal Mix	-25°C to -15°C
1	ELB	Elution Buffer	2°C to 8°C
1	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Core Library Prep Box 1, Store at -25°C to -15°C

Quantity	Reagent	Description
1	ATL	A-Tailing Mix
1	CTA	A-Tailing Control
1	CTE	End Repair Control
1	CTL	Ligation Control
1	LIG	Ligation Mix
1	RSB	Resuspension Buffer
1	STL	Stop Ligation Buffer

Core Library Prep Box 2, Store at -25°C to -15°C

Quantity	Reagent	Description
1	PMM	PCR Master Mix
1	PPC	PCR Primer Cocktail
1	FSA	First Strand Synthesis Act D Mix
1	SMM	Second Strand Marking Master Mix

TruSeq Stranded Total RNA Library Prep (96 Samples)

A quantity of two of each box is included for the 96 sample workflow.

Library Prep Box 1, Store as specified

Quantity	Reagent	Description	Storage
1	DTE	CTE Dilution Tube	Room Remperature
1	DTA	CTA Dilution Tube	Room Remperature
1	DTL	CTL Dilution Tube	Room Remperature
2	RRB	rRNA Removal Beads	2°C to 8°C

Library Prep Ribo-Zero Box, Store as Specified

You receive two of one of the following boxes, depending on the workflow. The box also contains plate barcode labels.

Library Preparation H/M/R (48 Samples)

Quantity	Reagent	Description	Storage
2	RBB	rRNA Binding Buffer	-25°C to -15°C
2	RRM	rRNA Removal Mix	-25°C to -15°C
2	ELB	Elution Buffer	2°C to 8°C
2	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Library Preparation Gold (48 Samples)

Quantity	Reagent	Description	Storage
2	RBB	rRNA Binding Buffer	-25°C to -15°C
2	RRM G	rRNA Removal Mix - Gold	-25°C to -15°C
2	ELB	Elution Buffer	2°C to 8°C
2	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Library Preparation Plant (48 Samples)

Quantity	Reagent	Description	Storage
2	RBB	rRNA Binding Buffer	-25°C to -15°C
2	RRM P	rRNA Removal Mix - Plant	-25°C to -15°C
2	ELB	Elution Buffer	2°C to 8°C
2	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Library Preparation Globin (48 Samples)

Quantity	Reagent	Description	Storage
2	RBB	rRNA Binding Buffer	-25°C to -15°C
2	GRM	Globin Removal Mix	-25°C to -15°C
2	ELB	Elution Buffer	2°C to 8°C
2	EPH	Elute, Prime, Fragment High Mix	-25°C to -15°C

Core Library Prep Box 1, Store at -25°C to -15°C

Quantity	Reagent	Description
2	ATL	A-Tailing Mix
2	CTA	A-Tailing Control
2	CTE	End Repair Control
2	CTL	Ligation Control
2	LIG	Ligation Mix
2	RSB	Resuspension Buffer
2	STL	Stop Ligation Buffer

Core Library Prep Box 2, Store at -25°C to -15°C

Quantity	Reagent	Description
2	PMM	PCR Master Mix
2	PPC	PCR Primer Cocktail
2	FSA	First Strand Synthesis Act D Mix
2	SMM	Second Strand Marking Master Mix

For information about the Inline Control DNA provided in this kit, see the Inline Control DNA section of the the TruSeq Stranded Total RNA Reference Guide.

New Products

TruSeq Stranded Total RNA Support

Contents & Storage

TruSeq Stranded Total RNA Library Prep (48 Samples)

Library Prep Box 1, Store as specified

Library Prep Ribo-Zero Box, Store as Specified

Ribo-Zero H/M/R

Ribo-Zero Gold

Ribo-Zero Plant

Ribo-Zero Globin

Core Library Prep Box 1, Store at -25°C to -15°C

Core Library Prep Box 2, Store at -25°C to -15°C

TruSeq Stranded Total RNA Library Prep (96 Samples)

Library Prep Box 1, Store as specified

Library Prep Ribo-Zero Box, Store as Specified

Library Preparation H/M/R (48 Samples)

Library Preparation Gold (48 Samples)

Library Preparation Plant (48 Samples)

Library Preparation Globin (48 Samples)

Core Library Prep Box 1, Store at -25°C to -15°C

Core Library Prep Box 2, Store at -25°C to -15°C

AutoLoader 2.x

BeadArray Reader

BeadXpress Reader

cBot

cBot 2

HiScan

HiSeq 1000

HiSeq 1500

HiSeq 2000

HiSeq 2500

HiSeq 3000

HiSeq 4000

HiScanSQ

HiSeq X

iScan

iSeq 100

MiniSeq

MiSeq

MiSeqDx

MiSeq FGx

NextSeq 500

NextSeq 550

NextSeq 550Dx

NeoPrep Library Prep System

NovaSeq 6000

TruSeq Stranded Total RNA Support

Contents & Storage

TruSeq Stranded Total RNA Library Prep (48 Samples)

Library Prep Box 1, Store as specified

Library Prep Ribo-Zero Box, Store as Specified

Ribo-Zero H/M/R

Ribo-Zero Gold

Ribo-Zero Plant

Ribo-Zero Globin

Core Library Prep Box 1, Store at -25°C to -15°C

Core Library Prep Box 2, Store at -25°C to -15°C

TruSeq Stranded Total RNA Library Prep (96 Samples)

Library Prep Box 1, Store as specified

Library Prep Ribo-Zero Box, Store as Specified

Library Preparation H/M/R (48 Samples)

Library Preparation Gold (48 Samples)

Library Preparation Plant (48 Samples)

Library Preparation Globin (48 Samples)

Core Library Prep Box 1, Store at -25°C to -15°C

Core Library Prep Box 2, Store at -25°C to -15°C