Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
This video discusses how to choose the appropriate sequencing primers for your run. Since commonly used Illumina indexes, TruSeq™ and Nextera™, utilize different index primer site sequences, it's important that the correct primer is loaded into the HiSeq for sequencing.
This video offers tips and troubleshooting advice related to the HiSeq Instrument computer, including navigating the instrument's Port Manager, formatting hard drives, and setting up a second run.
This video offers tips on the mechanical and thermal systems of the HiSeq, as well as best practices on maintaining your instrument.
This video introduces the fluidics system. Topics include fluidics leaks, syringe pump function, and priming the HiSeq 2500 template loading station.
This video continues to share tips and tricks for the fluidics system started in Part 4 of the series. The video focuses on how to identify and remove blockages from the fluidics lines to ensure the HiSeq instrument is used at its highest capacity.
In this video, an Illumina Field Applications Scientist discusses the key steps in the Nextera library preparation process and provides some tips and tricks on how to achieve optimal results. Note that the best practices presented in this video do not apply to the Nextera™ DNA Flex Library Prep Kit.
In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
This video provides an overview of overclustering and how it can impact your sequencing data. An Illumina Field Applications Scientist shows you how to use Sequencing Analysis Viewer (SAV) to look for common symptoms of an overclustered HiSeq or MiSeq flow cell.
Why do variations and inconsistencies arise in quantitation for next-generation sequencing? An Illumina Field Applications Scientist discusses the differences in several quantitation methods.
In this video, an Illumina Field Applications Scientist lists some tips and tricks for getting more consistent quantitation and shares quantitation success stories from other Illumina users. By the end, you’ll have a better understanding of quantitation in your lab and tools to make sure it becomes a reliable and consistent part of your next-generation sequencing workflow.
An Illumina Field Applications Scientist describes what it means to sequence through the insert of your prepared library. We look at Bioanalyzer traces to assess the range of insert sizes in a library.
This video shows you how to use Sequencing Analysis Viewer (SAV) plots to determine if your sequencing insert is too short. We also explore how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.