Real Time PCR

Training Videos

Online Course Title Description Length
Absolute Quantification

This course defines the key points to serial dilutions and unknown samples, how to perform a serial dilution, and demonstrates how to interpret a standard curve.

 

10 min
Absolute Quantification Data Analysis and Troubleshooting

This course describes data analysis steps and outline the various data abnormalities, and how to troubleshoot.

 

10 min
Are Your Samples Ready?

The course explains nucleic acid isolation workflows, defines different types of isolation methods, the two priming methods for reverse transcription, and proper handling and storage conditions

 

15 min
Eco Installation and Use, Part 1: Installing Eco

After completing this lesson you will be able to identify all Eco components and understand how to install the instrument and computer.

 

15 min
Eco Installation and Use, Part 2: Preparing and Loading the Evaluation Plate

After completing this lesson you will be able to get started using Eco using the evaluation plate, launch an experiment using the instrument software, and locate and identify output files.

 

15 min
Eco Installation and Use, Part 3: Data Analysis

After completing this lesson you will be able to define parameters for validating the evaluation plate experiment and improve your data quality.

 

15 min
Eco Multiplexing

This course defines multiplexing, its benefits, challenges, outlines the three modes, and details the steps to optimize assays.

 

10 min
Eco Real-Time PCR System Overview

This course provides an instrument overview including hardware components, instrument control software, applications supported by Eco software.

 

10 min
High Resolution Melt: Methylation Screening

This course defines high resolution melt (HRM), basic guidelines for assay design, samples, and PCR conditions; and the steps in the HRM workflow for methylation.

 

10 min
High Resolution Melt: Mutation Screening

This course defines high resolution melt (HRM), basic guidelines for assay design, samples, and PCR conditions; and the steps in the HRM workflow.

 

10 min
High Resolution Melt: SNP Genotyping

This course defines high resolution melt (HRM); several classes of SNPs, basic guidelines for assay design, samples, and PCR conditions; and the steps in the HRM workflow.

 

10 min
How Do I Set Up an Experiment in the Eco Software?

This course defines and explains how to set-up relative quantification, absolute quantification, SNP genotyping by allelic discrimination, high resolution melt, an multiplexing experiments.

 

30 min
Real-Time PCR (qPCR)

This course discusses the advantages of quantification PCR (qPCR), the fundamentals of the qPCR process, and describes how Eco brings qPCR technology into the reach of every researcher.

 

20 min
Relative Quantification: Efficiency Correction

This course describes three relative gene expression methods, defines the efficiency correction method, how to set up an efficiency correction method plate, and the mathematical steps for the efficiency correction method

 

10 min
Relative Quantification: Relative Standard Curve Method

This course describes three relative gene expression methods, relative standard curve method basics, setting up a plate layout, and performing the mathematical steps for the relative standard curve method.

 

5 min
Relative Quantification: Validation Experiments

This course introduces three validation experiments, describes the importance of each experiment, and defines the workflow and outcomes.

 

20 min
What is Real-Time PCR?

This course defines real-time PCR, names several real-time PCR applications, describes the similarities and differences between traditional PCR and real-time PCR, and defines basic steps in real-time PCR workflow.

 

5 min