Online Sequencing Courses

Training Videos

Online Course Title Description Length
Sequencing: Illumina Technology

This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run.

Languages: Spanish, Chinese (Simplified)

30 min
Sequencing: Illumina's Dual Indexing Strategy

The goal of dual index sequencing is to increase the multiplex level of sequencing per lane, so that more samples can be sequenced on the same flow cell.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
NovaSeq Series: Preparing for Your Install

This course introduces lab requirements, computing requirements, and delivery considerations for the NovaSeq Series. It also discusses the consumables required for running and maintaining the instrument.

15 min
NovaSeq Series: How to Start a Run

This course introduces the steps to start a NovaSeq sequencing run, prepare the consumables, and use the NovaSeq Control Software.

20 min
NovaSeq Series: System Overview

This course introduces the features of the NovaSeq Series, the steps in the sequencing workflow, and the NovaSeq sequencing technology.

20 min
SureCell WTA 3' Library Prep: Best Practices

This course provides an introduction to the SureCell WTA 3' Library Prep Kit and identifies the steps of the workflow that require attention to obtain optimal library preparation results.

20 min
HiSeq 1500/2500: Best Practices

This course covers best practices for your HiSeq 1500/2500. Topics covered include: instrument mode changes and washes, best practices for performing a HiSeq sequencing run, BaseSpace Sequence Hub and data compression options for your HiSeq runs, fluidics and flow cell plate care, and how to prepare the instrument for idling or shutdown.

 

25 min
HiSeq: Rapid Run Mode

This course describes the Rapid Run Mode available for HiSeq 2500 or HiSeq 1500. It identifies what is needed to perform a Rapid Run, describes the options for clustering the 2-lane flow cell, and lists ways to reduce data storage requirements for your HiSeq runs.

 

15 min
ExAmp Cluster Amplification Workflow

This course discusses the purpose of ExAmp cluster amplification, lists the steps in the ExAmp cluster amplification workflow, and provides best practices for preparing the ExAmp reaction.

20 min
NextSeq 500: System Overview

By the end of this course, you will be able to: identify features of the NextSeq 500 system, describe NextSeq 500 sequencing technology, and describe the simplified sequencing workflow of the NextSeq 500 system.

 

20 min
NextSeq: Preparing for Your Install

This course provides an overview of the key components of the NextSeq Series instruments, as well as the lab, computing, and electrical requirements for installation.

 

15 min
NextSeq: How to Start a Run

By the end of this course, you will be able to: identify the steps to start a run on the NextSeq, describe how to prepare the reagent cartridge, and start a sequencing run using NCS.

 

20 min
NextSeq: Does My Run Look Good?

This course introduces three options for monitoring your NextSeq run. It also describes key metrics in NextSeq Control Software (NCS), Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.

10 min
MiSeq: Introduction to the MiSeq System

After completing this course, you will be able to describe the simplified sequencing workflow on the MiSeq, understand the features of MiSeq, and list the six MiSeq sequencing workflows.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: Sequencing Fundamentals

After completing this course you will be able to describe DNA, RNA and the central dogma; discuss traditional sequencing methodologies; and compare traditional sequencing to Illumina's sequencing chemistry.

Languages: Spanish, Japanese

15 min
MiSeq: Sequencing Chemistry

After completing this course you will be able to describe the Illumina sequencing workflow, discuss the clustering process, and define the sequencing process.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: Getting Started

After completing this course you will be able to describe the lab environment required to use MiSeq, list key components of MiSeq, and list the user-supplied consumables required for running and maintaining MiSeq.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: How to Start a Run

After completing this course you will be able to list the library construction methods available for MiSeq, create an electronic sample sheet, prepare and load MiSeq reagents, and start a sequencing run using MiSeq Control Software (MCS).

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: MiSeq Reporter

After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows.

Languages: Spanish, Chinese (Simplified), Japanese

25 min
MiSeq: Data Analysis Overview

After completing this course you will be able to list the MiSeq data analysis tools, briefly describe the function of each tool, and list some considerations when choosing your MiSeq data analysis tool.

Languages: SpanishJapanese

10 min
MiSeq: Does My Run Look Good?

After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV).

10 min
MiSeq: Instrument Washes

This course provides an overview of the three MiSeq instrument washes: the post-run wash, the maintenance wash, and the standby wash.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
MiSeq: Imaging and Base Calling

After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls.  These processes are completed automatically by the MiSeq.

Languages: Spanish, Chinese (Simplified), Japanese

35 min
MiSeqDx: Illumina Worklist Manager

This course provides an overview of Illumina Worklist Manager (IWM). Topics covered include: the purpose of IWM, how to view or edit the default file repositories or locate version information, and the steps to create or edit a worklist.

 

15 min
MiSeqDx: Preparing for Your Install

After completing this course you will be able to describe the lab environment required to use the MiSeqDx instrument, list key components of the MiSeqDx instrument, and list the user-supplied consumables required for running and maintaining the MiSeqDx instrument.

 

15 min
MiSeqDx: How to Start a Run

After completing this course you will be able to describe how to create a worklist, list the library construction methods available for MiSeqDx, identify how to prepare and load MiSeqDx reagents, and start a sequencing run using MiSeq Operating Software (MOS).

 

20 min
MiSeqDx: MiSeq Reporter

After completing this course you will be able to identify and navigate the MiSeq Reporter interface for the MiSeqDx. You will also be able to understand the role of the secondary analysis tool (MiSeq Reporter) in data processing.

 

25 min
MiSeqDx: Did My CF Assay Work?

After completing this course you will be able to describe sample performance, identify where to find sample performance information in the MiSeq Reporter Details tab or the MiSeqDx analysis results file, and describe next steps based on sample performance.

 

15 min
MiSeqDx: Illumina User Management

The Illumina User Management (IUM) course details how the user names, passwords, and access levels are managed for the MiSeqDx instrument.

 

10 min
MiniSeq: System Overview

By the end of this course, you will be able to identify features of the MiniSeq System, describe MiniSeq sequencing technology, and describe the simplified sequencing workflow of the MiniSeq System.

20 min
MiniSeq: Preparing for Your Install

By the end of this course, you will be able to list the key components of the MiniSeq System, describe the lab environment required to install and use the MiniSeq System, and identify the user-supplied consumables required for running and maintaining the instrument.

15 min
MiniSeq: How to Start a Run

This course provides an overview of how to set up and start a sequencing run on the MiniSeq System.

20 min
NeoPrep: System Overview

This course provides an introduction to the NeoPrep Library Prep System and how it works.

15 min
NeoPrep: Preparing for Your Install

This course provides an overview of the lab environment and requirements for the NeoPrep Library Prep System.

10 min
NeoPrep: Performing a Run

This course provides an introduction to the steps to set up a NeoPrep run and the steps to collect your libraries after the run completes.

25 min
BaseSpace Sequence Hub: Introduction

The course explores the BaseSpace Sequence Hub interface and its key features.

20 min
BaseSpace Sequence Hub: FAQs

This course helps you to locate answers to commonly asked questions about BaseSpace Sequence Hub.

25 min
Preparing Runs with BaseSpace Sequence Hub

This course provides an overview of how to use the BaseSpace Sequence Hub Prep tab to enter sample information and parameters for your sequencing or NeoPrep run.

25 min
Sequencing Analysis Viewer (SAV)

This course provides the basics on how to get started using Sequencing Analysis Viewer (SAV). By the end of this course, you will be able to: identify the purpose of SAV, list the steps to load data into SAV, and describe the tabs in SAV.

 

15 min
Local Run Manager Overview

This course provides an introduction to the Local Run Manager software. Topics include: its purpose, how to access the software, and the analysis workflows. It also shows the steps to set up a sequencing run and view analysis results.

15 min
Illumina Experiment Manager

This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

25 min
DesignStudio: TruSeq Targeted RNA Expression

After completing this course you will be able to create a project using DesignStudio Gene Expression.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
CASAVA 1.8

This course provides a CASAVA overview, explores secondary analysis inputs, demultiplexing, alignment, variant detection and counting, and additional resources.

60 min
Linux Introduction

This course provides a basic introduction to the Linux command line, including navigation, file management, and file processing, with a focus on commands that are useful for running Illumina data analysis software.

30 min
Sample Prep: PCR Clean-Up

By the end of this course, you will be able to describe the PCR clean-up process.

 

10 min
Sample Prep: Library Normalization

By the end of this course, you will be able to describe the library normalization process.

 

10 min
Nextera DNA Sample Prep

After completing this course you will be able to describe the items necessary to run Nextera DNA sample preparation assay to generate MiSeq sequence-ready libraries, describe the steps in Nextera DNA sample preparation workflow, and identify some of the assay's most important considerations.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
Nextera Rapid Capture

After completing this course, you will be able to identify the Nextera Rapid Capture Enrichment kits, choose your Nextera Rapid Capture Enrichment Product, identify how the protocol works, describe what is needed to successfully complete the protocol, define the various steps in the Sample Preparation workflow, and analyze Nextera Capture Enrichment data.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
Nextera Sample Prep: Best Practices

After completing this course you will be able to determine appropriate starting material, perform critical techniques used in the sample prep process, and accurately validate the final libraries.

 

30 min
TruSeq Amplicon - Cancer Panel

By the end of this training, you will be able to describe the items necessary to run the TruSeq Amplicon Cancer Panel assay, describe the steps in the sequencing workflow, identify some of the best practices, and set up a sample sheet for sequencing.

 

20 min
TruSeq Synthetic Long-Read Library Prep Overview and Getting Started

This course provides an overview of the TruSeq Synthetic Long-Read DNA Library Prep kits by briefly introducing you to the kit contents, workflow, and required specialized user-supplied equipment. It is not to be considered a replacement for the designated user guide.

20 min
TruSeq Synthetic Long-Read DNA Library Prep Best Practices

This course outlines best practices and critical techniques used to perform the TruSeq Synthetic Long-Read DNA Library Preparation. Topics covered include: DNA input recommendations, step-by-step review of the workflow, and library validation techniques.

20 min
TruSeq Targeted RNA Expression Kit

After completing this course you will be able to describe the TruSeq Targeted RNA Expression Kit, the steps to order a custom oligo pool, the steps in the TruSeq Targeted RNA Expression protocol, best practices, and how to analyze and normalize data.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: Best Practices

After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: Comparison of Available DNA Sample Preparation Kits

After completing this course you will know the similarities and differences between currently available TruSeq DNA Sample Prep Kits, the starting input amounts and final library characteristics for the various kits, how the various protocols differ, and the ordering information for the various TruSeq DNA kits.

 

20 min
TruSeq: Controls

By the end of this course you will be able to identify controls, describe the different SAV reporting types, and understand how to support these controls.

Languages: Spanish, Chinese (Simplified), Japanese

15 min
TruSeq: DNA PCR-Free Library Prep

Topics included in this course include the TruSeq DNA PCR-Free library prep protocol, what the protocol does, how the protocol works, what is needed to complete the protocol successfully, and the various steps in the workflow.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: DNA Sample Prep

After completing this course you will know the TruSeq DNA Sample Prep Protocol, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the Sample Prep Workflow.

 

10 min
TruSeq: Nano DNA Sample Prep

After completing this course, you will know: what the TruSeq Nano DNA Sample Prep Protocol is, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the sample prep workflow.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: RNA Access Library Prep

By the end of this course you will learn what the TruSeq RNA Access Library Prep protocol is, how the protocol works, what is needed to successfully complete the protocol, the various steps in the sample preparation workflow, and how to analyze TruSeq RNA Access Library Prep data.

20 min
TruSeq: Sample Purification Bead Size Selection and Best Practices

After completing this course you will understand what Sample Purification Beads are, how the Sample Purification Beads work, when Sample Purification Beads are used, what the Size Selection Workflow is, and the Sample Purification Beads best practices.

 

20 min
TruSeq: Small RNA Sample Prep

After completing this course you will know the TruSeq Small RNA Sample Prep Protocol, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the Sample Prep Workflow.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
TruSeq: Stranded RNA Sample Prep

After completing this course, you will learn answers to questions including: What is the TruSeq Stranded mRNA Sample Prep Protocol? and What is the TruSeq Stranded Total RNA Sample Prep Protocol

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSight One Sequencing Panel

By the end of this training, you will be able to define the TruSight One Sequencing Panel, understand how the protocol works, identify what is needed to successfully complete the protocol, describe the various steps in the TruSight One workflow, and identify the tools used for analysis in TruSight One data.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
DesignStudio: TruSeq Custom Amplicon

This course provides an introduction to the DesignStudio application by walking the user through ordering a experiment.

Languages: Japanese

20 min
TruSeq: Custom Amplicon Library Prep

After completing this course you will be able to describe the items necessary to run the TruSeq Custom Amplicon assay, list the steps in the workflow, and identify the best practices.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq Exome Library Preparation

By the end of this course, you will be able to identify the TruSeq Exome kits, choose your TruSeq Exome product, identify how the protocol works, describe what is needed to complete the protocol, define the various steps in the library preparation and enrichment workflow, and analyze TruSeq Exome data.

20 min
TruSeq Rapid Exome

This course provides an overview of the TruSeq Rapid Exome library prep kit, including, how to choose a TruSeq Rapid Exome product, how the protocol works, what is needed to successfully complete the protocol, what are the various steps in the library preparation workflow and how to analyze TruSeq Rapid Exome data.

20 min
Nextera XT DNA Library Preparation

By the end of this course, you will be able to identify the Nextera XT DNA kits, identify how the protocol works, describe what is needed to complete the protocol, and define the various steps in the library preparation workflow.

20 min