Sequencing

This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

This course discusses the purpose of ExAmp cluster amplification, lists the steps in the ExAmp cluster amplification workflow, and provides best practices for preparing the ExAmp reaction.

This course describes the Rapid Run Mode available for HiSeq 2500 or HiSeq 1500. It identifies what is needed to perform a Rapid Run, describes the options for clustering the 2-lane flow cell, and lists ways to reduce data storage requirements for your HiSeq runs.

This course provides an overview of the DRAGEN analysis pipelines. By the end of this course, you will be able to identify the role of the DRAGEN Platform in secondary data analysis, list the available pipelines, and briefly describe the expected performance and accuracy.

This course provides an overview of the DRAGEN server installation process, lists the resources needed for the self-installation, and describes placement, security, and network considerations.

This course describes the benefits of using the DRAGEN Platform for secondary analysis of sequencing data, provides an introduction to the technology, and lists available pipelines.

Languages: Japanese

This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

This course provides an introduction to the Local Run Manager v1 software for MiniSeq instruments using MiniSeq Control Software v1. Topics include: its purpose, how to access the software, and the analysis workflows. It also shows the steps to set up a sequencing run and view analysis results.

This course shows you how to get started using the Local Run Manager v2 software to set up your sequencing runs and view analysis results. Local Run Manager v2 is available for MiniSeq instruments using MiniSeq Control Software v2 or later, NextSeq 500/550 instruments using NextSeq Control Software v4 and later, and MiSeq instruments using MiSeq Control Software v3.

Languages: French, Italian, German, Spanish, Chinese (Simplified)

After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV).

Languages: Chinese (Simplified), German, Japanese, Korean, Spanish

After completing this course you will be able to identify the MiSeq run setup options, describe how to prepare the MiSeq reagent cartridge and load libraries, and list the steps to start a sequencing run with MiSeq Control Software (MCS).

Languages: Chinese (Simplified), German, Japanese, Korean, Spanish

After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls.  These processes are completed automatically by the MiSeq.

After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows. MiSeq Reporter is available on instruments using MiSeq Control Software v2.6 and earlier.

After completing this course you will be able to describe the lab environment required to use the MiSeq, list key components of MiSeq, identify the network considerations for MiSeq, and list the user-supplied consumables required for running and maintaining your MiSeq.

Languages: Chinese (Simplified), German, Japanese, Korean, Spanish

This course explores the options for monitoring your MiniSeq run. Also detailed are the key metrics in MiniSeq Control Software, Sequencing Analysis Viewer, and BaseSpace Sequence Hub.

This course provides an overview of how to set up and start a sequencing run on the MiniSeq System.

By the end of this course, you will be able to list the key components of the MiniSeq System, describe the lab environment required to install and use the MiniSeq System, and identify the user-supplied consumables required for running and maintaining the instrument.

By the end of this course, you will be able to identify features of the MiniSeq System, describe MiniSeq sequencing technology, and describe the simplified sequencing workflow of the MiniSeq System.

This course introduces four options for monitoring your NextSeq 550 run. It also describes key metrics in NextSeq Control Software (NCS), Local Run Manager, Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.

By the end of this course, you will be able to: identify the steps to start a run on the NextSeq 550, describe how to prepare the reagent cartridge, and start a sequencing run using NCS.

This course provides an overview of the key components of the NextSeq 550 instrument, as well as the lab, computing, and electrical requirements for installation.

By the end of this course, you will be able to: identify features of the NextSeq 550 system, describe NextSeq sequencing technology, and describe the simplified sequencing workflow of the NextSeq system.

After completing this course, you will be able to identify the Nextera Rapid Capture Enrichment kits, choose your Nextera Rapid Capture Enrichment Product, identify how the protocol works, describe what is needed to successfully complete the protocol, define the various steps in the Sample Preparation workflow, and analyze Nextera Capture Enrichment data.

Languages: Spanish, Chinese (Simplified), Japanese

By the end of this course, you will be able to identify the Nextera XT DNA kits, identify how the protocol works, describe what is needed to complete the protocol, and define the various steps in the library preparation workflow.

This course introduces lab requirements, computing requirements, and delivery considerations for the NovaSeq 6000 System. It also discusses the consumables required for running and maintaining the instrument.

This video demonstrates the NovaSeq™ Xp workflow, including NovaSeq flow cell preparation, ExAmp and library master mix preparation, and manually loading libraries onto the flow cell.

This course provides the basics on how to get started using Sequencing Analysis Viewer (SAV). By the end of this course, you will be able to: identify the purpose of SAV, list the steps to load data into SAV, and describe the tabs in SAV.

This course provides an introduction to the SureCell WTA 3' Library Prep Kit and identifies the steps of the workflow that require attention to obtain optimal library preparation results.

After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

Topics included in this course include the TruSeq DNA PCR-Free library prep protocol, what the protocol does, how the protocol works, what is needed to complete the protocol successfully, and the various steps in the workflow.

After completing this course, you will know: what the TruSeq Nano DNA Library Protocol is, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the sample prep workflow.

By the end of this course you will learn what the TruSeq RNA Access Library Prep protocol is, how the protocol works, what is needed to successfully complete the protocol, the various steps in the sample preparation workflow, and how to analyze TruSeq RNA Access Library Prep data.

After completing this course you will understand what sample purification beads are, how the beads work, when the beads are used, what the size selection workflow is, and be able to list some best practices.

By the end of this course, you will be able to: identify the items needed for the protocol, describe the steps in the library prep protocol, describe the steps in the enrichment protocol, and list best practices for performing the protocol.

This course discusses general guidelines for assessing your run’s performance.

This course introduces the steps to start an iSeq sequencing run, prepare the consumables, and use the iSeq Control Software.

Languages: Chinese (Simplified), German, French, Italian, Spanish

By the end of this course, you will be able to: prepare your lab for the arrival and installation of the iSeq 100 system, unpack and install the instrument, identify the test components required to perform first time setup, and perform first time setup of your instrument.

Languages: French, Italian, German, Spanish, Chinese (Simplified)