Canvas (Copy Number Variant Caller)

Canvas is an algorithm for calling copy number variants from a diploid sample. Most of a normal DNA sample is diploid, or having 2 copies. Canvas identifies regions of the sample genome that are not present, or present either one time or more than 2 times in the genome. Canvas scans the genome for regions having an unexpected number of short read alignments. Regions with fewer than the expected number of alignments are classified as losses. Regions having more than the expected number of alignments are classified as gains.

Canvas is appropriately applied to low-depth cytogenetics experiments, low-depth single-cell experiments, or whole-genome sequencing experiments. Canvas is not appropriate for whole exome experiments, cancer studies, or any other experiment with the following conditions:

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Most of the genome is not assumed to be diploid.

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Reads are not distributed randomly across the diploid genome.

For Research Use Only. Not for use in diagnostic procedures.