Introduction | Workflow Requirements

Workflow Requirements

▶ This app supports the following:
▶ Human genomes.
▶ Samples with paired-end reads.
▶ BAM output files from Isaac Whole Genome Sequencing v4 AppResults as inputs.
▶ Read lengths that are greater than or equal to 32 bp.
▶ Read lengths is between 100 and 150 bases. Reads shorter than 50 bases result in a warning, while reads shorter than 32 bases cause an error in the app.
▶ Minimum normal sample data set size is 150 gigabases.
▶ Approximately 375 million reads assuming 2 × 100.
▶ Approximately 250 million reads assuming 2 × 150.
▶ Minimum tumor sample data set size is 300 gigabases.
▶ Approximately 750 million reads assuming 2 × 100.
▶ Approximately 500 million reads assuming 2 × 150.
▶ Maximum combined (normal+tumor) data set size is 650 gigabases.
▶ Approximately 3.25 billion reads assuming 2 × 100.
▶ Approximately 2.17 billion reads assuming 2 × 150.
▶ This app does not support mate pair sample or other non-forward and -reverse styles of paired-end sequencing.