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Isaac Somatic Variant Caller
The Isaac Somatic Variant Caller detects somatic SNVs and indels in sequencing data from a tumor and matched normal sample, based on the following assumptions:
| ▶ | The normal sample is a mixture of diploid germline variation and noise. |
| ▶ | The tumor sample is a combination of the normal sample and somatic variation. It is assumed that the somatic variation and the normal noise can occur at any allele frequency ratio. |
For SNVs, but not for indels, the normal noise component is further modeled as a combination of single-strand and double-strand noise.
Figure Isaac Somatic Variant Caller Method
Note
For a detailed overview of Isaac Somatic Variant Caller methods, go to www.ncbi.nlm.nih.gov/pubmed/22581179.