Infinium HTS iSelect Methyl Custom BeadChip Contents & Storage

MA1

1 tube

Room temperature

LX1

1 tube

-15° to -25°C

LX2

1 tube

-15° to -25°C

EML

1 tube

-15° to -25°C

RA1

-15° to -25°C

FMS

1 tube

-15° to -25°C

RPM

1 tube

-15° to -25°C

MSM

1 tube

-15° to -25°C

XC3

1 bottle

Room temperature

XC4

1 bottle

Room temperature

SML

1 tube

-15° to -25°C

PM1

1 tube

2° to 8°C

PB2

1 tube

Room temperature

Te-Flow Flow-Through Chambers (with Black Frames,
Spacers, Glass Back Plates, and Clamps)

1 (per BeadChip)

Room temperature

Multi-Sample BeadChip Alignment Fixture

1 (per 8 BeadChips)

Room temperature

Hyb Chamber inserts

8

Room temperature

Wash Dish

2 (up to 8 BeadChips)

Room temperature

Wash Rack

1 (up to 8 BeadChips)

Room temperature

Alconox Powder Detergent

as needed

Room temperature

EtOH

as needed

Room temperature

Hyb Chambers

2

Room temperature

Hyb Chamber gaskets

2

Room temperature

BeadChips

2

2° to 8°C

95% formamide/1 mM EDTA

15 ml

-15° to -25°C

BCD plate with Bisulfite-converted DNA samples

1 plate

-15° to -25°C

Lambda DNA

See Instructions

2° to 8°C

0.1N NaOH

15 ml (per 96 samples)

2° to 8°C

PicoGreen dsDNA quantitation reagent

See Instructions

2° to 8°C

Zymo Lightning Methylation kit (includes bisulfite-conversion reagent,
dilution buffer, binding buffer, desulphonation buffer, elution buffer)

D5046: 4x96 samples (manual)

or

D5047: 8x96 samples (manual)

or

D5049: 1x96 samples (automation)

Room temperature

Fluotrac 200 96-well flat-bottom plate

1 per Std DNA plate

1 per Sample DNA plate

Room temperature

Genomic DNA (for HTS Methylation)

500 ng for each bisulfite conversion reaction for manual process

1000 ng for each bisulfite conversion reaction for the Automated process

Room temperature

EtOH

500 ml

Room temperature

96-well 0.2 ml skirted microplate

1–3 plates

Room temperature

1X TE

See Instructions

Room temperature

96-well 0.8 ml microtiter plate (MIDI)

1 plate

Room temperature

100% 2-propanol

40 ml (per 96 samples)

Room temperature

96-well 0.65 ml microtiter plate

1 per 96 samples

Room temperature