Request a Lab Requeue
Biosamples that have not produced the requested amount of yield are marked as Missing Yield. Use a Lab Requeue to request more sequencing yield from the lab. After the requeue request is set to Acknowledged, incoming yield is applied to the request. For more information about yield calculations, see Yield.
A lab requeue requests additional biosample data. For information about requeueing a run with a fixed sample sheet, see Fix Sample Sheet.
The lab requeue returns the biosample to one of three stages in the lab preparation process.
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Biosample—The original biosample is prepared in a new library for sequencing. |
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Library—An existing library is used for sequencing. |
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Pool—An existing pool is used for sequencing. |
You can track the status or cancel the requeue from the Requeues tab of the biosample details page.
Requeue a Biosample
Request a Biosample Requeue if there were problems with the sequencing data from a biosample, or the existing library or pool does not have enough volume to produce the required yield. This request initiates a new library preparation from the original biosample.
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Open the biosample details page for the biosample you want to requeue. |
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Select the Summary tab. |
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Select the File drop-down arrow, point to Requeue, and then select Biosample. |
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In the Requeue Biosample dialog, specify the requeue parameters. |
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Requested yield (Gbp/Mbp) |
Requeue a Library
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Open the biosample details page for the biosample you want to requeue. |
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Select the Libraries tab. |
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Select the checkbox for the library you want to requeue. You can only requeue one library at a time. |
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Select the File drop-down arrow, point to Lab Requeue, and then select Library. |
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In the Requeue Library dialog, specify the requeue parameters. |
Requeue a Pool
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Open the biosample details page for the biosample you want to requeue. |
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Select the Librariestab. |
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Select the pool name for the desired library. If more than one pool exists for the library, select Pools, and then select the desired pool from the list. |
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Select the File drop-down arrow, point to Lab Requeue, and then select Pool. |
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In the Requeue Pool dialog, specify the requeue parameters. |
Acknowledge a Lab Requeue Request
Pending Lab Requeues are listed the Requeues tab of the Biosample Details page. When you have determined that your lab can fulfill the request, change the status to Acknowledged.
For information about acknowledging requeues using the BaseSpace Sequence Hub API, see the developer documentation at developer.basespace.illumina.com.
NOTE
If the requeue is not acknowledged, incoming yield is applied to the original prep request.
Cancel a Requeue
You can cancel a pending requeue. You might do this if the requeue is not needed, or there were errors in the requeue.
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Open the biosample details page for the requeued biosample. |
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Select the Requeues tab. |
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To cancel a library, do the following. |
- Select a library.
- Select the Status drop-down arrow, point to Cancel, and then select Library Requeue
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To cancel a pool, do the following. |
- Select a pool.
- Select the Status drop-down arrow, point to Cancel, and then select Pool Requeue