HiSeq 3000 System Support
The HiSeq 3000 System has been discontinued. Illumina will support the instrument and intends to supply the reagents through February 28, 2023. To find an alternative instrument, use the Platform Comparison Page.
Documentation
Software Downloads
Tools
View AllCustom Protocol Selector
Generate end-to-end documentation tailored to your experiment.
Library Prep and Array Kit Selector
Determine the best kit for your project type, starting material, and method or application.
Sequencing Coverage Calculator
Determine reagents and sequencing runs for your desired coverage.
Training
View AllMaximize the effectiveness of your Illumina system, train new employees, or learn the latest techniques and best practices. We offer support webinars, online courses, expert video tips, and instructor-led trainings.
Featured Training
ExAmp Cluster Amplification Workflow
Discusses the purpose of ExAmp cluster amplification, lists the steps in the workflow, and provides best practices.
HiSeq 3000/4000: Introduction
Overview of the HiSeq 3000/4000 workflow, patterned flow cell technology, ExAmp clustering, and best practices.
HiSeq Expert Video Tips Part 5: Removing Fluidic Line Blockages
How to identify and remove blockages from the fluidics lines to ensure the HiSeq instrument is used at its highest capacity.