Contents & Storage

The AmpliSeq for Illumina Focus protocol requires AmpliSeq for Illumina Focus Panel (Catalog # 20019164), AmpliSeq for Illumina Library PLUS (Catalog # 20019101, 20019102, or 20019103), and AmpliSeq for Illumina CD Indexes Set A (96 indexes, 96 samples) (Catalog # 20019105).  The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.

The AmpliSeq for Illumina Focus Panel contains 2 oligo pools, 1 for RNA and 1 for DNA, which can be processed separately or together. The folowing table shows required kit quantities and assumes that DNA and RNA libraries come from the same sample. 

Number of Samples*

24 Samples

96 Samples 384  Samples
Number of DNA and RNA Libraries† 48
(24 DNA + 24 RNA)
192
(96 DNA + 96 RNA)
768
(384 DNA + 384 RNA)
AmpliSeq for Illumina Focus Panel 1 4 16
AmpliSeq Library PLUS For Illumina 2 x 24-reaction kit (20019101) 2 x 96-reaction kit (20019102) 2 x 384-reaction kit (20019103)
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 2 8
cDNA Synthesis 1 1 4

*A sample is a specimen of nucleic acid from an individual source. Samples can be DNA, RNA, or Paired. For the purposes of this table, we assume that samples are paired. 

†A library is nucleic acids from a sample after preparation and contains the required ends for sequencing. Since DNA and RNA are prepared separately, each preparation is considered a unique library. Therefore, a single sample can give rise to 2 libaries (1 for DNA and 1 for RNA).

AmpliSeq for Illumina Focus Panel, Store at -25°C to -15°C*

Quantity

Reagent

   
1 5X AmpliSeq Focus DNA Panel Pool 1    
1 5X AmpliSeq Focus RNA Panel Pool 1    

*Ships at room temperature, but must be stored at -25°C to -15°C

AmpliSeq Library PLUS for Illumina Contents, Store at -25°C to -15°C

 

Tube Quantity for Kit Configuration

24-reaction

96-reaction

384-reaction Reagent
1 4 16 1X Lib Amp Mix
1 1 4 10X Library Amp Primers
1 1 4 DNA Ligase
1 1 4 5X AmpliSeq HiFi Mix
1 1 4 FuPa Reagent
1 2 8 Low TE**
1 1 4 Switch Solution

**Can be stored at room temperature

AmpliSeq for Illumina CD Indexes Set A, Store at -25°C to -15°C*

Quantity

Description

1 AmpliSeq CD Indexes Set A plate (96 indexes, 96 samples)

*Ships at room temperature, but must be stored at -25°C to -15°C

AmpliSeq Library Equalizer for Illumina

Quantity

Description

Storage
1 Equalizer Beads 2°C to 8°C
1 Equalizer Capture 2°C to 8°C
1 Equalizer Elution Buffer 2°C to 8°C
1 Equalizer Wash Buffer 15°C to 30°C
1 Equalizer Primer 2°C to 8°C