Illumina DNA Prep with Enrichment Training

This course describes the Nextera Flex for Enrichment technology, identifies the items needed, defines the various steps in the library preparation and enrichment steps, and provides an overview for sequencing and analysis.

After completing this course, you will be able to create an Illumina Custom Enrichment panel in DesignStudio. This course provides an introduction to DesignStudio and Illumina Custom Enrichment panels, guides you through the steps of creating and ordering a custom enrichment panel, and provides additional resources to get you started.

Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.

Recorded Webinar (September 2020) | Illumina offers several enrichment-based library prep kits for targeted resequencing. This webinar will deconstruct enrichment data analysis for beginners. We will focus on the following topics: Illumina Enrichment analysis options, enrichment analysis workflow, enrichment analysis output files, and what to look for when assessing enrichment output data.

Recorded Webinar (August 2020) | Join us to learn more about our newest and most efficient enrichment workflow – Illumina DNA Prep with Enrichment, formerly known as Nextera Flex for Enrichment. This webinar is for users that are interested in targeted sequencing using enrichment with fixed and custom gene panels, as well as for current users of Illumina enrichment workflows to learn and compare the benefits Illumina DNA Prep with Enrichment offers. We discuss the improved enrichment workflow used in Illumina DNA Prep with Enrichment, the unique features of Illumina DNA Prep with Enrichment, and useful tips for a successful library prep.  

CORRECTION: There is an update for low minor allele frequency (somatic variant) detection. Illumina has validated only 1-plex pooling for somatic variant detection. If you would like to pool above 1-plex for this application, you will need to validate the performance of higher plexity pooling.

Illumina Field Applications Scientists review individual best practices and troubleshooting for the Illumina DNA Prep with Enrichment – Tagmentation protocol (formerly known as Nextera Flex for Enrichment). Guidance and recommended best practices are provided for both the Library Preparation and Enrichment portions of the protocol.

Upcoming Webinar | (Note: 11:00 AM CET) Est-ce que ma librairie est bonne pour être séquencée ? C'est quoi ce pic ? Ce webinaire expliquera comment utiliser le BioAnalyzer d’Agilent pour vérifier la qualité de votre librairie avant le séquençage et comment identifier des éventuels problèmes lors de la préparation des échantillons. Ce webinaire s'adresse aux utilisateurs débutants et intermédiaires et couvrira les sujets suivants: comment identifier les caractéristiques d'une trace de librairie idéale, comment reconnaître les problèmes potentiels, comment prévenir les problèmes potentiels

Upcoming Webinar | (Note: 11:00 AM CET) Sieht meine Library für die Sequenzierung gut aus? Was ist das für ein Peak? In diesem Webinar erfahren Sie, wie Sie den Agilent BioAnalyzer zur Überprüfung der Libraryqualität vor der Sequenzierung und zur Fehlersuche bei der Probenvorbereitung einsetzen können. Dieses Webinar richtet sich an neue und fortgeschrittene Benutzer und behandelt folgende Themen: Wie erkenne ich die Charakteristik idealer Library Traces, Wie erkenne ich mögliche Probleme, Wie kann ich mögliche Probleme vermeiden

Upcoming Webinar | (Note: 11:00 AM CET) La mia libreria ha un bell'aspetto per poter essere sequenziata? Cos'è quel picco? Questo seminario online tratterà come utilizzare l' Agilent BioAnalyzer per controllare la qualità della libreria prima del sequenziamento e per risolvere i problemi relativi alla preparazione dei campioni. Questo seminario online è rivolto a nuovi utenti e con media esperienza e tratterà i seguenti argomenti: come identificare le caratteristiche di una traccia ideale di libreria, come riconoscere potenziali problemi, come prevenire potenziali problemi

Upcoming Webinar | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.