This guide contains everything a first-time user needs, including an overview of the Nextera DNA Flex protocol, tips and techniques, protocol steps, kit contents, and user-supplied consumables.
This checklist is a condensed form of the Nextera DNA Flex protocol. It is easy to print and carry with you in the lab.
Consumables and equipment used with the Nextera DNA Flex Kit. Download this form to a preferred location before using it. The interactivity in this form is not designed for use in a browser window.
Instructions for preparing samples from Dried Blood Spot cards.
This quick reference card describes how to set the adapter trimming sequences for the Nextera DNA Flex library prep kit specifically on sequencing runs using an Illumina MiniSeq.
The Index Adapters Pooling Guide provides guidelines for preparing libraries with balanced index combinations for sequencing on Illumina systems. This guide replaces the TruSeq Library Prep Pooling Guide (document # 15042173) and the Nextera Low Plex Pooling Guidelines (Pub. No. 770-2011-044).
Instructions for preparing crude lysate from bacterial culture and stool samples.
Instructions for preparing samples from bacteria colonies.