Catalog IDs: 20025523, 20025524
Nextera Flex for Enrichment is a fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole exome enrichment using oligo panels from Illumina and third party vendors meeting specified requirements.
Enrichment probe panels and IDT for Illumina Nextera UD Indexes are sold separately.
This protocol is compatible with double-stranded high-quality gDNA input from 10 ng to 1000 ng, extracted FFPE input from 50 ng to 1000 ng, blood samples processed with the Flex Lysis Reagent Kit and Blood Lysis protocol, and saliva samples processed with the Saliva Lysis protocol.
|Sample Input Type||Quantification of Input DNA Required||Required DNA Input Quantity||Normalized Pre-Enriched Library Yield|
|10–49 ng genomic DNA||Yes||260/280 ratio of 1.8–2.0||No|
|50–1000 ng genomic DNA||No||260/280 ratio of 1.8–2.0||Yes|
|50–1000 ng extracted FFPE||Yes||∆ Cq value ≤ 5||No|