When clustering libraries on the cBot or cBot 2 Systems and sequencing on the HiSeq 1000 or HiSeq 2000 Systems or in High Output mode on the HiSeq 1500 or HiSeq 2500 Systems, new primers are required whether performing a non-indexed, single-indexed, or dual-indexed run.
Use the TruSeq Dual Index Sequencing Primer Box for Paired End runs (catalog # PE-121-1003), which contains the required primers for sequencing (HP10, HP11, HP12).
Quantifying a Nextera Rapid Capture Enrichment post-enriched library using a fluorometric method results in the following cluster densities. Results can vary based on your method of quantification.
|Instrument||Mode/Version||Expected Cluster Density (mm2)|
|NextSeq||High Output Flowcell||200,000-240,000|
|Mid Output Flowcell||200,000-240,000|
* Used for Nextera Rapid Capture Custom Enrichment only