This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
Recorded Webinar (July 2019) | Part 1 of a 4-Part RNA-Seq Webinar Series. This session describes key considerations when planning RNA sequencing experiments. We will cover RNA sample preparation through sequencing to basic data analysis. We assume a basic understanding of Illumina sequencing technology, but no prior knowledge of RNA sequencing is required. The webinar is targeted towards both new and experienced users.
Recorded Webinar (August 2019) | Part 2 of a 4-part RNA-Seq webinar series. This session will review sample preparation kit options for total RNA, mRNA, and targeted RNA sequencing projects. Great for both new and experienced users, we will assume a basic understanding of Illumina sequencing technology, although no knowledge of sample prep is required. In particular, we’ll discuss: how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.
Recorded Webinar (August 2019) | Part 4 of a 4-Part RNA-Seq Webinar Series. In this webinar, we will discuss the RNA-Seq analysis options available on BaseSpace including the RNA-Seq Alignment/Cufflinks, TopHat/Cufflinks, and RNA Express apps. We assume both a basic understanding of Illumina sequencing technology and knowledge of the general library prep workflow for the TruSeq RNA kits. The webinar is targeted towards both new and experienced users. Please note that small RNA sequencing analysis is not be covered in this webinar. We encourage you to view Part I: Introduction, Part II: Kits and Part III: Best Practices, prior to this webinar.
Recorded Webinar (August 2019) | Part 3 of a 4-part RNA-Seq webinar series. In the third part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s TruSeq RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, and TruSeq Stranded Total RNA and discuss: Bead handling, quantification methods, library QC, and troubleshooting.
Recorded Webinar (December 2019) | FFPE is a widely used process to conserve tissues and generally contain partially degraded or limited genomic material. Specific considerations are required to sequence these challenging samples. This webinar is targeted towards both new and experienced users, with a basic knowledge of working with FFPE samples and will go over the following topics: A basic introduction to FFPE sample types, the nucleic acid extraction process, and library Preparation options.