TruSeq RNA Exome Training

This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

Recorded Webinar (October 2021) | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.

Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.

Recorded Webinar (August 2021) | Part 1 of a 3-Part RNA-Seq Webinar Series. This session will review sample preparation kit options for RNA input.  An introduction to the new Illumina Stranded mRNA Ligation, Illumina Stranded Total RNA Ligation with Ribozero Plus, and Illumina RNA prep with Enrichment Tagmentation workflows will be provided along with a comparison to established Illumina kits. This webinar is targeted towards both new and experienced users and will cover the following topics: key considerations when planning RNA sequencing experiments, how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.

Recorded Webinar (September 2021) | In the second part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, Illumina Stranded mRNA Ligation, TruSeq Stranded Total RNA, Illumina Stranded Total RNA Ligation and Illumina RNA prep with Enrichment Tagmentation. We will cover the following topics: bead handling, quantification methods, library QC and troubleshooting.

Recorded Webinar (August 2019) | Part 3 of a 3-Part RNA-Seq Webinar Series. In this webinar, we will discuss the RNA-Seq analysis options available on BaseSpace including the RNA-Seq Alignment/Cufflinks, TopHat/Cufflinks, and RNA Express apps. We assume both a basic understanding of Illumina sequencing technology and knowledge of the general library prep workflow for the TruSeq RNA kits. The webinar is targeted towards both new and experienced users. Please note that small RNA sequencing analysis is not be covered in this webinar. We encourage you to view Part I: Introduction to Illumina’s RNA library preparation workflows and Part II: Best practices for Illumina’s RNA Prep protocols, prior to this webinar.

Recorded Webinar (January 2021) | FFPE is a widely used process to conserve tissues and generally contain partially degraded or limited genomic material. Specific considerations are required to sequence these challenging samples. This webinar is targeted towards both new and experienced users, with a basic knowledge of working with FFPE samples and will go over the following topics: a basic introduction to FFPE sample types, the nucleic acid extraction process, and library Preparation options.