This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
Recorded Webinar (October 2021) | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.
Recorded Webinar (May 2016) | Part 1 of a 2-part Small RNA-Seq Webinar series. In this webinar we describe key considerations when planning small RNA sequencing experiments. We cover RNA isolation, library preparation, sequencing and data analysis. We assume a basic understanding of Illumina sequencing technology, but no knowledge of library preparation is required. The webinar is targeted towards both new and experienced users, and will include a question and answer session. We encourage you to view the second part of this series; Part II: Best Practices.
Recorded Webinar (June 2016) | Part 2 of the the 2-part Small RNA-Seq webinar series. In this webinar we discuss important best practices for generating successful small RNA libraries with the TruSeq Small RNA library preparation kit, and provide advice on assessing input material and evaluating final libraries. We assume a basic understanding of Illumina sequencing technology, but no knowledge of library preparation is required. The webinar is targeted towards both new and experienced users, and will include a question and answer session. We encourage you to attend Small RNA Sequencing Part I: Introduction before this webinar.