TruSeq Stranded mRNA Library Prep Kit for NeoPrep Compatible Products

The NeoPrep System normalizes libraries to 10nM. Use the following table to determine insert, concentration, and load size for your system and the resulting expected cluster density. 

• Dilute the normalized library to the predenature concentration.
• Denature and dilute the library to the intermediate concentration. Depending on the application, pooling after denaturation and dilution can increase library complexity due to the number of unique molecules present in the final mixture when each library is denatured separately in a larger volume.
• Load the denatured samples using the pooled denatured samples load size.