TruSeq Amplicon - Cancer Panel Training

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  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min

  • TruSeq: Best Practices

    After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

    20 min

  • How Do I Optimize Amplicon Sequencing Data? Part 1

    What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.

    6 min

  • How Do I Optimize Amplicon Sequencing Data? Part 2

    An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.

    7 min

  • Preventing Contamination

    Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.

    10 min

  • Amplicon Sequencing: Assessing the success of your run

    Recorded Webinar (June 2021) | Illumina invites you to join us to discuss evaluation of amplicon sequencing. This webinar is targeted for new and intermediate users of Illumina Next Generation Sequencing. In this webinar, we will review sequencing run performance for different Illumina amplicon sequencing workflows, including AmpliSeq and 16S, and analysis results. Our discussion will cover the following: Optimal sequencing run set up; Evaluating sequencing performance; Supplementary analysis files; Amplicon analysis options; Key analysis metrics

  • Amplicon Sequencing: Introduction

    Recorded Webinar (May 2021)| Illumina Technical Support invites you to join us to discuss the end-to-end workflow for an amplicon sequencing project. This webinar is ideal for new users of Illumina Next Generation Sequencing. We will discuss amplicon sequencing, including the following topics: what defines an amplicon library, kits and protocols for amplicon libraries, and amplicon sequencing runs and parameters.

  • Sequencing FFPE Samples: Introduction

    Recorded Webinar (January 2021) | FFPE is a widely used process to conserve tissues and generally contain partially degraded or limited genomic material. Specific considerations are required to sequence these challenging samples. This webinar is targeted towards both new and experienced users, with a basic knowledge of working with FFPE samples and will go over the following topics: a basic introduction to FFPE sample types, the nucleic acid extraction process, and library Preparation options.

  • Library QC and Troubleshooting with the BioAnalyzer - EMEA (French)

    Upcoming Webinar | Est-ce que ma librairie est bonne pour être séquencée ? C'est quoi ce pic ? Ce webinaire expliquera comment utiliser le BioAnalyzer d’Agilent pour vérifier la qualité de votre librairie avant le séquençage et comment identifier des éventuels problèmes lors de la préparation des échantillons. Ce webinaire s'adresse aux utilisateurs débutants et intermédiaires et couvrira les sujets suivants: comment identifier les caractéristiques d'une trace de librairie idéale, comment reconnaître les problèmes potentiels, comment prévenir les problèmes potentiels

    Oct 14, 2021 GMT

  • Library QC and Troubleshooting with the BioAnalyzer - EMEA (German)

    Upcoming Webinar | Sieht meine Library für die Sequenzierung gut aus? Was ist das für ein Peak? In diesem Webinar erfahren Sie, wie Sie den Agilent BioAnalyzer zur Überprüfung der Libraryqualität vor der Sequenzierung und zur Fehlersuche bei der Probenvorbereitung einsetzen können. Dieses Webinar richtet sich an neue und fortgeschrittene Benutzer und behandelt folgende Themen: Wie erkenne ich die Charakteristik idealer Library Traces, Wie erkenne ich mögliche Probleme, Wie kann ich mögliche Probleme vermeiden

    Oct 21, 2021 GMT

  • Library QC and Troubleshooting with the BioAnalyzer - EMEA (Italian)

    Upcoming Webinar | La mia libreria ha un bell'aspetto per poter essere sequenziata? Cos'è quel picco? Questo seminario online tratterà come utilizzare l' Agilent BioAnalyzer per controllare la qualità della libreria prima del sequenziamento e per risolvere i problemi relativi alla preparazione dei campioni. Questo seminario online è rivolto a nuovi utenti e con media esperienza e tratterà i seguenti argomenti: come identificare le caratteristiche di una traccia ideale di libreria, come riconoscere potenziali problemi, come prevenire potenziali problemi

    Nov 04, 2021 GMT