TruSeq RNA Sample Prep Kit Training

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  • TruSeq: Best Practices

    After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

    Languages: Spanish, Chinese (Simplified), Japanese

    20 min


  • RNA Sequencing: Part I - Introduction

    Recorded Webinar (July 2019) | Part 1 of a 4-Part RNA-Seq Webinar Series. This session describes key considerations when planning RNA sequencing experiments. We will cover RNA sample preparation through sequencing to basic data analysis. We assume a basic understanding of Illumina sequencing technology, but no prior knowledge of RNA sequencing is required. The webinar is targeted towards both new and experienced users.


  • RNA Sequencing: Part II - Kits

    Recorded Webinar (August 2019) | Part 2 of a 4-part RNA-Seq webinar series. This session will review sample preparation kit options for total RNA, mRNA, and targeted RNA sequencing projects. Great for both new and experienced users, we will assume a basic understanding of Illumina sequencing technology, although no knowledge of sample prep is required. In particular, we’ll discuss: how to identify which kit is right for your experiment, what are the expected input and outputs, and where to find additional resources.


  • RNA Sequencing: Part IV - Introduction to Analysis

    Recorded Webinar (August 2019) | Part 4 of a 4-Part RNA-Seq Webinar Series. In this webinar, we will discuss the RNA-Seq analysis options available on BaseSpace including the RNA-Seq Alignment/Cufflinks, TopHat/Cufflinks, and RNA Express apps. We assume both a basic understanding of Illumina sequencing technology and knowledge of the general library prep workflow for the TruSeq RNA kits. The webinar is targeted towards both new and experienced users. Please note that small RNA sequencing analysis is not be covered in this webinar. We encourage you to view Part I: Introduction, Part II: Kits and Part III: Best Practices, prior to this webinar.


  • RNA Sequencing: Part III - Best Practices

    Recorded Webinar (August 2019) | Part 3 of a 4-part RNA-Seq webinar series. In the third part of our RNA-Seq Webinar series, we dive into best practices for Illumina’s TruSeq RNA kits. This webinar is aimed at both new and experienced users with a basic understanding of Illumina Sequencing technology and workflow. We will focus on TruSeq RNA v2, TruSeq Stranded mRNA, and TruSeq Stranded Total RNA and discuss: Bead handling, quantification methods, library QC, and troubleshooting.