TruSight Oncology 500 High Throughput Compatible Products

Compatible Sequencing Systems

Compatible Analysis Software

The TSO500 Local App v2.1 is compatible with v1 sequencing consumables.

The TSO500 Local App v2.2 is compatible with both v1 and v1.5 sequencing consumables. 

Read Lengths for the TruSight Oncology 500 High Throughput Kit

Read/Index Number of cycles
Read 1 101
‌Index 1 10
Index 2 10
Read 2 101

Analysis of TSO 500 HT Data Using TSO 500 v2.1 Local App Using Multiple Nodes

This table is based on 72 paired DNA/RNA samples from a NovaSeq S2 flow cell.
Samples per Node Total Turn Around Time (Hours)* AWS Instance Type Instance vCPU Instance Memory (GiB)
8 3.67 c5_9xlarge 36 72
16 6.76 c5_9xlarge 36 72
24 10.17 c5_9xlarge 36 72
32 13.68 c5_9xlarge 36 72
48 21.00 c5_9xlarge 36 72
72 30.60 c5_9xlarge 36 72
8 1.73 c5D_18xlarge 72 144
16 3.23 c5D_18xlarge 72 144
24 6.87 c5D_18xlarge 72 144
32 9.20 c5D_18xlarge 72 144
48 14.47 c5D_18xlarge 72 144
72 23.33 c5D_18xlarge 72 144

*Post demultiplexing. Average demultiplexing time is 3-4 hours for a NovaSeq S2 flow cell.

Number of Libraries and Selecting Indexes

For optimal coverage, use the following guidelines for the number of libraries per sequencing run.

NovaSeq 6000
Flow Cell Minimum Maximum
SP

8 DNA

8 RNA

16 DNA

16 RNA

S1

16 DNA

16 RNA

32 DNA

32 RNA

S2

32 DNA

32 RNA

72 DNA

72 RNA

S4

72 DNA

72 RNA

192 DNA

192 RNA

NextSeq 500, NextSeq 550, NextSeq 550Dx in RUO Mode
Flow Cell Minimum Maximum
HO

3 DNA

3 RNA

8 DNA

8 RNA