DRAGEN TSO 500 Analysis Software v1.1.
The TSO 500 Local App v2.1 is compatible with v1 sequencing consumables.
The TSO 500 Local App v2.2 is compatible with both v1 and v1.5 sequencing consumables.
| Read/Index | Number of cycles |
|---|---|
| Read 1 | 101 |
| Index 1 | 10 |
| Index 2 | 10 |
| Read 2 | 101 |
| Samples per single DRAGEN server | Total Turn Around Time (Hours) |
|---|---|
| 8 tissue biopsy samples | 2 hours |
| 16 tissue biopsy samples | 3 hours |
| 32 tissue biopsy samples | 5 hours |
| 16 tissue biopsy samples | 10 hours |
| Samples per Node | Total Turn Around Time (Hours)* | AWS Instance Type | Instance vCPU | Instance Memory (GiB) |
|---|---|---|---|---|
| 8 | 3.67 | c5_9xlarge | 36 | 72 |
| 16 | 6.76 | c5_9xlarge | 36 | 72 |
| 24 | 10.17 | c5_9xlarge | 36 | 72 |
| 32 | 13.68 | c5_9xlarge | 36 | 72 |
| 48 | 21.00 | c5_9xlarge | 36 | 72 |
| 72 | 30.60 | c5_9xlarge | 36 | 72 |
| 8 | 1.73 | c5D_18xlarge | 72 | 144 |
| 16 | 3.23 | c5D_18xlarge | 72 | 144 |
| 24 | 6.87 | c5D_18xlarge | 72 | 144 |
| 32 | 9.20 | c5D_18xlarge | 72 | 144 |
| 48 | 14.47 | c5D_18xlarge | 72 | 144 |
| 72 | 23.33 | c5D_18xlarge | 72 | 144 |
*Post demultiplexing. Average demultiplexing time is 3-4 hours for a NovaSeq S2 flow cell.
For optimal coverage, use the following guidelines for the number of libraries per sequencing run.
| Flow Cell | Minimum | Maximum |
|---|---|---|
| SP | 8 DNA 8 RNA |
16 DNA 16 RNA |
| S1 | 16 DNA 16 RNA |
32 DNA 32 RNA |
| S2 | 32 DNA 32 RNA |
72 DNA 72 RNA |
| S4 | 72 DNA 72 RNA |
192 DNA 192 RNA |
| Flow Cell | Minimum | Maximum |
|---|---|---|
| HO | 3 DNA 3 RNA |
8 DNA 8 RNA |