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TruSight Oncology 500 v2 Contents & Storage

 

TSO 500 v2 RNA Library Prep ‑20C, Store at -25°C to -15°C
24 Manual  32 Auto  96 Auto  Reagent Description
1 1 2 EPH Elute, Prime, Fragment High Mix 3
1 1 2 FSM First Strand Synthesis Mix 
1 1 2 RVT Reverse Transcriptase
1 1 2 SSM Second Strand Mix
TSO 500 v2 Library Prep 4C, See Storage Temperatures in Table
24 Manual 48 Manual 32 Auto 64 Auto 96 Auto Reagent Description Storage
1 1 2 1 4 IPB Illumina Purification Beads Room Temperature
1 1 2 1 4 RSB Resuspension Buffer 2°C to 8°C
1 1 1 1 1 TEB TE Buffer 2°C to 8°C
TSO 500 v2 Library Prep ‑20C, Store at ‑25°C to ‑15°C
24 Manual 48 Manual 32 Auto 64 Auto 96 Auto Reagent Description
2 2 1 1 2 ATL4 A-Tailing Mix
2 2 1 2 2 EPM4 Enhanced PCR Mix 4
2 2 1 1 2 ERP6 End Repair 6
2 2 1 1 2 LIGX Ligation Mix
1 1 1 1 2 STL Stop Ligation Buffer
1 1 1 1 2 UMI3 UMI Index Anchors 3
TSO 500 v2 Enrichment 4C, See Storage Temperatures in Table
24 Manual 48 Manual 32 Auto 64 Auto 96 Auto Reagents Description Storage
1 1 1 1 2 EHB2 Enrich Hyb Buffer 2 2°C to 8°C
1 1 1 1 2 HP3 2N NaOH 2°C to 8°C
1 1 1 1 2 IPB Illumina Purification Beads Room Temperature
1 1 1 1 2 LNB3 Library Normalization Beads 3 2°C to 8°C
2 2 1 1 2 LNW1 Library Normalization Wash 1 2°C to 8°C
1 1 1 1 2 RSB Resuspension Buffer 2°C to 8°C
2 2 1 1 2 SMB4 Streptavidin Magnetic Beads 4 2°C to 8°C
TSO 500 v2 Enrichment ‑20C (DNA), Store at -25°C to -15°C
48 Manual 64 Auto Reagent Description
2 1 EE1 Enrichment Elution 1
1 2 EEW Enhanced Enrichment Wash
2 1 EPM4 Enhanced PCR Mix 4
1 1 LNA1 Library Normalization Additives 1
1 1 LNS1 Library Normalization Storage Buffer 1
2 1 NHB2 Hyb Buffer 2 + IDT NXT Blockers
2 2 OPD2 Oncology DNA Probe Pool 2
2 2 OPD3 Oncology DNA Probe Pool 3
3 1 PPC3 PCR Primer Cocktail 3
TSO 500 v2 Enrichment ‑20C (DNA/RNA), Store at -25°C to -15°C
 
24 Manual 32 Auto 96 Auto Reagent Description
2 1 2 EE1 Enrichment Elution 1
1 2 4 EEW Enhanced Enrichment Wash
2 1 2 EPM4 Enhanced PCR Mix 4
1 1 2 LNA1 Library Normalization Additives 1
1 1 2 LNS1 Library Normalization Storage Buffer 1
2 1 2 NHB2 Hyb Buffer 2 + IDT NXT Blockers
1 2 2 OPD2 Oncology DNA Probe Pool 2
1 2 2 OPD3 Oncology DNA Probe Pool 3
1 1 2 OPR1 Oncology RNA Probe Pool 1
3 1 2 PPC3 PCR Primer Cocktail 3
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