VeriSeq PGS Library Preparation Kit Contents & Storage

The VeriSeq PGS Kit contains two SurePlex DNA Amplification System Kits, one VeriSeq Library Prep Kit– PGS, one VeriSeq Index Kit– PGS, and four MiSeq Reagent Kits v3– PGS. Components are shipped on dry ice unless specified otherwise below. As soon as you receive your kit, store the kit components at the specified temperature in designated pre-amplification and post-amplification areas.

SurePlex DNA Amplification System Kit, Store at -25°C to -15°C

Reagent
Cell Extraction Buffer
Extraction Enzyme Dilution Buffer
Cell Extraction Enzyme
SurePlex Pre-Amp Enzyme
SurePlex Amplification Buffer
SurePlex Amplifcation Enzyme

VeriSeq Library Preparation Kit–PGS

Box 1, Freeze until use
Reagent Description Storage
ATM Amplicon Tagment Mix, 96 RXN -25°C to -15°C
TD Tagment DNA Buffer -25°C to -15°C
NPM Nextera PCR Master Mix -25°C to -15°C
RSB Resuspension Buffer -25°C to -15°C
LNA1 Library Normalization Additives 1 -25°C to -15°C
LNW1 Librart Normalization Wash 1 2°C to 8°C
HT1 Hybridization Buffer -25°C to -15°C
Box 2, Refrigerate until use
Reagent Description Storage
NT Neutralize Tagment Buffer Room temperature
LNB1 Library Normalization Buffer 2°C to 8°C
LNS1 Library Normalization Storage Buffer 1 Room temperature

VeriSeq Index Kit– PGS (96 samples)

Quantity Component Storage
4 tubes Index Primers, S503, S504 -25°C to -15°C
12 tubes Index Primers, N701 to N712 -25°C to -15°C
MiSeq Reagent Kit v3–PGS

Box 1

Quantity Component Storage
1 Reagent Cartridge -25°C to -15°C
1 HT1 -25°C to -15°C

Box 2

Quantity Component Storage
1 PR2 Bottle 2°C to 8°C
1 MiSeq Flow Cell 2°C to 8°C